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Chromosome Engineering Techniques Modify Contents and Constituents of Fructans in Cultivated Allium Species
http://hdl.handle.net/10659/1770
http://hdl.handle.net/10659/1770c763077d-164c-4a06-abfc-633362ee46ed
名前 / ファイル | ライセンス | アクション |
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S-926_Onodera.pdf (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-03-31 | |||||
タイトル | ||||||
タイトル | Chromosome Engineering Techniques Modify Contents and Constituents of Fructans in Cultivated Allium Species | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Japanese bunching onion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mapping | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | onion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | shallot | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | sucrose | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yaguchi, Shigenori
× Yaguchi, Shigenori× McCallum, John× Shaw, Martin× Pither-Joyce, Meeghan× Hang, Tran Thi Minh× Tsukazaki, Hikaru× Hoa, Vu Quynh× Masuzaki, Shin-ichi× Wako, Tadayuki× Onodera, Shuichi× Shiomi, Norio× Yamauchi, Naoki× Shigyo, Masayoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Onion (Allium cepa L.) and shallot (A. cepa Aggregatum group) exhibit wide variation in bulb fructan content and the Frc locus on chromosome 8 conditions much of this variation. To understand the biochemical basis of Frc we conducted biochemical and genetic analyses of Allium fistulosum (FF) - shallot alien monosomic addition lines (AALs; FF+1 A-FF+8A), onion mapping populations and shallot - A. fistulosum addition lines. Sucrose and fructan levels in leaves of FF+2A were significantly lower than FF throughout the year. FF+8A showed significantly higher winter sucrose accumulation and sucrose phosphate synthase (SPS) activity. Markers for additional candidate genes for sucrose metabolism were obtained by cloning a major SPS expressed in onion leaf and exhaustively mining onion EST resources. SPS and sucrose synthase (SuSy) loci were assigned to chromosome 8 and 6 respectively using AALs and linkage mapping. Further loci were assigned, using AALs, to chromosomes 1 (sucrose phosphate phosphatase), 2 (SuSy and 3 invertases) and 8 (neutral invertase). The shallot - A. fistulosum AAL (AA+8F) also showed the high fructan accumulation. The concordance between chromosome 8 localization of SPS and elevated leaf sucrose levels conditioned by high fructan alleles at the Frc locus in bulb onion or alien monosomic additions of chromosome 8 in A. fistulosum and in A. cepa suggest that the Frc locus may condition variation in SPS activity. | |||||
書誌情報 |
Dynamic Biochemistry, Process Biotechnology and Molecular Biology 巻 3, 号 Special Issue 1, p. 70-77, 発行日 2009-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1749-0626 | |||||
権利(URI) | ||||||
権利情報 | http://www.globalsciencebooks.info/Journals/DBPBMB.html | http://www.globalsciencebooks.info/Journals/DBPBMB.html | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Global Science Books Ltd. | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |