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Crucial factor causing collapse and aggregation of cultured cells in epon resin
http://hdl.handle.net/10659/4305
http://hdl.handle.net/10659/4305251f5242-907d-48f2-8f3e-91d55aee3b61
名前 / ファイル | ライセンス | アクション |
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S-2015-214_takehana.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-05-23 | |||||
タイトル | ||||||
タイトル | Crucial factor causing collapse and aggregation of cultured cells in epon resin | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | electoron microscopy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cultured cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | epon resin embedding | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
TANGKAWATTANA, Prasarn
× TANGKAWATTANA, Prasarn× YAMAGUCHI, Mamoru× KLOMKLEAW, Wuthichai× NIU, Hua× MINAGUCHI, Jun× TAKEHANA, Kazushige |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Ultrastructural artifacts regarding collapse and aggregation of cultured cells have been problematic, especially when investigated apoptotic cells. The infiltration process during sample preparation is considered to be the most crucial factor for this problem. This study was conducted using two culture systems: a suspension culture system of human T-lymphocyte Jurkat cells and rabbit mature dendritic cells and a monolayer culture system of human lung macrophages, human breast cancer cells (A-546 cells) and cat bone-invasive gingival cancer cells (sccf3 cells). Fixation was conducted prior to removing or detaching the cells from the culture dishes. Initial infiltration with a 1 : 3 volume ratio of epon resin : propylene oxide was found to be the most crucial step among these cultured cells. The improved epon-resin infiltration method could eliminate the artifacts. Thus, differentiation between artifactual images and true images is highly possible. | |||||
書誌情報 |
Okajimas Folia Anatomica Japonica 巻 91, 号 1, p. 1-4, 発行日 2014-05 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0030-154X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2535/ofaj.91.1 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | オカジマ・フォリア アナトミカ・ヤポニカ編集部 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |