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Effect of Escherichia coli and Staphylococcus aureus on the In vitro Response of Peripheral Blood Mononuclear Cells Isolated from Holstein Dairy Cows had Ketosis or Milk Fever of Field Cases
http://hdl.handle.net/10659/4294
http://hdl.handle.net/10659/42946d5643bf-53a8-48c5-a87c-3eb646c14db3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
S-2015-186_ohtsuka.pdf (180.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2016-05-23 | |||||
| タイトル | ||||||
| タイトル | Effect of Escherichia coli and Staphylococcus aureus on the In vitro Response of Peripheral Blood Mononuclear Cells Isolated from Holstein Dairy Cows had Ketosis or Milk Fever of Field Cases | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cytokine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | dairy cows | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | immune function | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ketosis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | milk fever | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Ohtsuka, H.
× Ohtsuka, H.× Kanikawa, N.× Kudo, K.× Kyutoku, F.× Kakinuma, S.× Kohno, M.× Maeda, Y.× Tanabe, T. |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The expression of cytokines mRNA, including Interleukin (IL)-2, IL-4 and Interferon (IFN)-γ and lymphocyte proliferation stimulated in vitro with either Escherichia coli (E. coli) or Staphylococcus aureus (S.A.) by Peripheral Blood Mononuclear Cells (PBMC) obtained from Holstein dairy cows were examined after the onset of ketosis or milk fever of field cases during the lactation period. The cows were used 50 to 150 days after calving in this study and divided into three groups; seventeen cows with milk fever (MF Group, 94.9±8.6 days after calving), ten cows with ketosis (Ketosis Group, 96.3±10.2 days after calving) and twenty-seven cows with no clinical diseases (Control Group, 89.4±5.4 days after calving). PBMC were stimulated in vitro by soluble antigen of E. coli or S.A. together with recombinant IL-2. The numbers of the peripheral blood T cells and monocyte were lower in the Ketosis Group in comparison with those in the Control Group and a significant difference was found in the number of CD3^+TcR1-N12^+T cells. There was a significantly lower lymphocyte proliferation stimulated with E. coli and S.A. in the Ketosis Group compared to that in the Control Group. Expression of IL-2, IL-4 and IFN-γ mRNA in PBMC stimulated with S.A. in the Ketosis Group was significantly lower than that in the Control Group, but these cytokine expression showed no group differences when PBMC were stimulated with E. coli. The present study demonstrated the onset of ketosis during the lactation period decreases reactivity of PBMC at least when they were stimulated with S.A in vitro. | |||||
| 書誌情報 |
International Journal of Animal and Veterinary Advances 巻 6, 号 4, p. 116-121, 発行日 2014-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2041-2894 | |||||
| 権利 | ||||||
| 権利情報 | H. Ohtsuka, N. Kanikawa, K. Kudo, F. Kyutoku, S. Kakinuma, M. Kohno, Y. Maeda and T. Tanabe, 2014. Effect of Escherichia coli and Staphylococcus aureus on the In vitro Response of Peripheral Blood Mononuclear Cells Isolated from Holstein Dairy Cows had Ketosis or Milk Fever of Field Cases. International Journal of Animal and Veterinary Advances, 6(4): 116-121. | |||||
| 権利（URI） | ||||||
| 権利情報 | http://creativecommons.org/licenses/by/3.0/ | http://creativecommons.org/licenses/by/3.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | MAXWELL Scientific Publication | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
