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Effects of NF-κB Expression and Its Inhibition on Canine Mammary Cancer Cell Lines in an Immunodeficient Mice Model
http://hdl.handle.net/10659/3255
http://hdl.handle.net/10659/3255d731a99e-9878-4e36-9cc2-c7c76ba29279
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-06-04 | |||||
| タイトル | ||||||
| タイトル | Effects of NF-κB Expression and Its Inhibition on Canine Mammary Cancer Cell Lines in an Immunodeficient Mice Model | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | canine MGT | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | metastasis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | molecular targeted therapy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NF-κB | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | xenografted model | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
MKAOUAR, Lobna
× MKAOUAR, Lobna× ENDO, Yoshifumi× MOCHIZUKI, Manabu× NISHIMURA, Ryohei× SASAKI, Nobuo× NAKAGAWA, Takayuki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Two canine mammary gland tumor (MGT) cell lines, CHMp-5b and -13a, were transplanted into nude mice, and their tumor development and metastatic potential were evaluated. In addition, NF-κB, Ki-67 and cyclin D1 expressions in the tumor masses were evaluated, and the relationship between these expressions and malignancy of the tumors developed in nude mice was investigated. Lower activation of NF-κB was positively correlated with a lower potential of metastasis and better prognosis in nude mice xenografted with CHMp-13a compared with CHMp-5b. Then, CHMp-5b cells were treated with BAY11-7082, an inhibitor of NF-κB, and the expressions of I-κBα, p-I-κBα, cyclin D1 and Bcl-2 in these cells were evaluated by Western blot analysis. In addition, BAY11-7082 at a dose of 6 mg/kg was administered intraperitoneally to nude mice xenografted with CHMp-5b, and relationships between tumor growth and lung and lymph node metastasis in nude mice and NF-κB, I-κBα and p-I-κBα expressions in tissues of these mice were evaluated. BAY11-7082 treatment induced decreased expressions of p-I-κBα, cyclin D1 and Bcl-2 in a dose- and time-dependent manner, suggesting that BAY inhibited NF-κB in this cell line. CHMp-5b-xenografted mice treated with BAY11-7082 showed a reduction in tumor growth and metastasis. Therefore, inhibition of the NF-κB signaling pathway may be a promising novel therapeutic approach for canine MGTs. | |||||
| 書誌情報 |
The journal of veterinary medical science 巻 73, 号 12, p. 1539-1546, 発行日 2011-12 |
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| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1292/jvms.10-0580 | |||||
| 出版者 | ||||||
| 出版者 | 日本獣医学会 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
