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Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D
http://hdl.handle.net/10659/3037
http://hdl.handle.net/10659/3037f2f70093-7305-439a-ab0a-0dfb3fb1bd16
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-06-04 | |||||
タイトル | ||||||
タイトル | Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Sasaki, Michihito
× Sasaki, Michihito× Hasebe, Rie× Makino, Yoshinori× Suzuki, Tadaki× Fukushi, Hideto× Okamoto, Minoru× Matsuda, Kazuya× Taniyama, Hiroyuki× Sawa, Hirofumi× Kimura, Takashi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The endotheliotropism of equine herpesvirus-1 (EHV-1) leads to encephalomyelitis secondary to vasculitis and thrombosis in the infected horse central nervous system (CNS). To identify the host factors involved in EHV-1 infection of CNS endothelial cells, we performed functional cloning using an equine brain microvascular endothelial cell cDNA library. Exogenous expression of equine major histocompatibility complex (MHC) class I heavy chain genes conferred susceptibility to EHV-1 infection in mouse NIH3T3 cells, which are not naturally susceptible to EHV-1 infection. Equine MHC class I molecules bound to EHV-1 glycoprotein D (gD), and both anti-gD antibodies and a soluble form of gD blocked viral entry into NIH3T3 cells stably expressing the equine MHC class I heavy chain gene (3T3-A68 cells). Treatment with an anti-equine MHC class I monoclonal antibody blocked EHV-1 entry into 3T3-A68 cells, equine dermis (E. Derm) cells and equine brain microvascular endothelial cells. In addition, inhibition of cell surface expression of MHC class I molecules in E. Derm cells drastically reduced their susceptibility to EHV-1 infection. These results suggest that equine MHC class I is a functional gD receptor that plays a pivotal role in EHV-1 entry into equine cells. | |||||
書誌情報 |
Genes to Cells 巻 16, 号 4, p. 343-357, 発行日 2011-04 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1356-9597 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/j.1365-2443.2011.01491.x | |||||
出版者 | ||||||
出版者 | Molecular Biology Society of Japan / Blackwell Publishing Ltd | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |