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A simple model system for identifying arbuscular mycorrhizal fungal taxa that actively colonize rice (Oryza sativa L.) roots grown in field soil
http://hdl.handle.net/10659/00005986
http://hdl.handle.net/10659/00005986f7057479-4d54-42ea-8b2e-cf1dc638cbc3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
R-2018-36_kobae.pdf (2.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-08-08 | |||||
| タイトル | ||||||
| タイトル | A simple model system for identifying arbuscular mycorrhizal fungal taxa that actively colonize rice (Oryza sativa L.) roots grown in field soil | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | active colonization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | arbuscular mycorrhizas | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | fungal taxa | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | infection unit | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Oryza sativa | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | vital staining | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | active colonization | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | arbuscular mycorrhizas | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | fungal taxa | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | infection unit | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Oryza sativa | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | vital staining | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
小八重, 善裕
× 小八重, 善裕× Ohtomo, Ryo× Oka, Norikuni× Morimoto, Sho |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Even a few centimeters of roots in field soils can be colonized by genetically diverse arbuscular mycorrhizal (AM) fungi. The DNA sequences of AM fungi in roots suggest the fungal identities; however, it is difficult to determine which AM fungal taxa are physiologically active. In this study, we took advantage of the characteristics of rice (Oryza sativa L.) mycorrhizal roots, in which active colonization in roots is easily detected via histochemical staining of fungal succinate dehydrogenase activity (vital staining) and individual active colonization regions (infection units) in roots rarely coalesce. Root segments (< 3 mm) containing an active infection unit were dissected and squashed, large subunit (LSU) ribosomal RNA genes (rDNAs) were amplified using fungal universal primers and the sequences were directly determined by Sanger sequencing. All obtained sequences of colonization regions were of glomeromycotan origin. Phylogenetic analysis revealed that the levels of LSU-rDNA heterogeneity within an active colonization site were different among different clades. The methodology presented in this study offers researchers a novel tool for investigating the DNA information of physiologically active AM fungi in roots, whereas the factors that affect genetic diversity among active colonization remain to be clarified. | |||||
| 書誌情報 |
Soil Science and Plant Nutrition 巻 63, 号 1, p. 29-36, 発行日 2017 |
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| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1080/00380768.2016.1277156 | |||||
| 権利 | ||||||
| 権利情報 | This is an Accepted Manuscript of an article published by Taylor & Francis in Soil Science and Plant Nutrition on 2017, available at https://www.tandfonline.com/doi/full/10.1080/00380768.2016.1277156 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 出版者 | ||||||
| 出版者 | Taylor & Francis Group | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
