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Performance evaluation of a newly designed on-farm blood testing system for determining blood non-esterified fatty acid and β-hydroxybutyrate concentrations in dairy cows
http://hdl.handle.net/10659/00007009
http://hdl.handle.net/10659/0000700950d33a68-285a-4775-b1b1-9ccf3da663e3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
R-2021-74_oikawa.pdf (306.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-04-01 | |||||
| タイトル | ||||||
| タイトル | Performance evaluation of a newly designed on-farm blood testing system for determining blood non-esterified fatty acid and β-hydroxybutyrate concentrations in dairy cows | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Dairy cow | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Non-esterified fatty acids | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | β-Hydroxybutyrate | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | On-farm blood testing | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Dairy cow | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Non-esterified fatty acids | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | β-Hydroxybutyrate | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | On-farm blood testing | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
FUKUMORI, Rika
× FUKUMORI, Rika× Taguchi, Takayuki× Oetzel, Garrett R.× 及川, 伸 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The objective of this study was to evaluate a newly designed on-farm blood testing system (OFBTS) for monitoring blood concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) in dairy cows. Blood samples from 230 Holstein dairy cows between −86 and 343 days in milk were collected. A drop of whole blood was used to determine NEFA and BHBA using the OFBTS. Plasma from the remaining blood was used to determine both analytes using a commercial kit (gold standard). In the repeatability of the OFBTS, the intra-assay CV for NEFA and BHBA were 1.3% and 4.5%, and the inter-assay CV were 1.8% and 2.9%, respectively. The slope and coefficient of determination of OFBTS analysis of NEFA compared to the gold standard were 0.92 and 0.94. Those for BHBA were 0.94 and 0.98. Mean of the difference between the gold standard laboratory assays and OFBTS of NEFA and BHBA were 0.021 and 0.019, respectively. However, the bias became substantial for NEFA in the higher concentration ranges (>1.2 mEq/L). The sensitivity and specificity of NEFA were 93.2% and 99.4% at a cutpoint of 0.4 mEq/L, and 87.9% and 100% at 0.6 mEq/L. Those of BHBA were 86.2% and 99.0% at a cutpoint of 1.0 mM, and 94.7% and 99.5% at 1.2 mM. The reaction time for the NEFA to reach 0.6 mEq/L was 7 min. The BHBA reaction reached 1.2 mM within 2 min. In conclusion, the OFBTS has excellent performance for evaluating blood NEFA or BHBA concentrations. | |||||
| 書誌情報 |
Research in Veterinary Science 巻 135, p. 247-252, 発行日 2021-03 |
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| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.rvsc.2020.09.011 | |||||
| 権利 | ||||||
| 権利情報 | © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. | |||||
| 権利（URI） | ||||||
| 権利情報 | http://creativecommons.org/licenses/by-nc-nd/4.0/ | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 出版者 | ||||||
| 出版者 | Elsevier Ltd. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
