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The antioxidant activity of the Maillard reaction product, β-LG-xylobiose, was measured in vitro and compared to that of conjugated β-LG-lactose. The reaction for 7 days led to conjugated β-LG-xylobiose with a relative molecular mass ranging between 19 and 22 kDa based on SDS-PAGE analysis. It is confirmed that xylobiose bound to β-LG by gas-liquid chromatography. One milligram of conjugated β-LG-xylobiose contains 108 μg of xylobiose, while the available ε-amino group content decreased to 40% after the Maillard reaction. Conjugated β-LG-xylobiose had a higher radical scavenging activity than free β-LG. As the modification by xylobiose had a higher efficiency than the modification by lactose, it is found that xylobiose is a useful oligosaccharide for protein modification. Furthermore, the radical scavenging activity of β-LG was improved by modification with xylobiose. メイラード反応を利用してβ-lactoglobulin (β-LG)に,xylobioseを導入した.得られたβ-LG-xylobioseのin vitroにおける抗酸化活性をβ-LG-lactoseと比較検討した.7日間の反応を行ったところ,β-LG-xylobioseの分子量はSDS-PAGE分析により19-22 kDaと推定され,MALDI TOF-MS分析から約21 kDaを中心に多く分布していることがわかった.ガスクロマトグラフィー分析を行ったところ,xylobioseがβ-LGに結合していることが確認された.また,β-LG-xylobiose 1 mg当たり108 μgのxylobioseが結合したと算出され,遊離ε-アミノ基は約40%に減少したと算出された.DPPHを用いたラジカル捕捉能試験の結果より,β-LG-xylobioseはβ-LGよりも高いラジカル捕捉能を有していることが明らかになり,β-LG-lactoseのラジカル捕捉能よりも高かった.この結果より,xylobioseはタンパク質の糖化修飾における修飾糖として適したオリゴ糖と考えられる.以上の結果より,メイラード反応を利用したxylobiose糖化修飾によってβ-LGのラジカル捕捉能が向上することが確認された.", "subitem_description_type": "Abstract"}]}, "item_2_publisher_36": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本応用糖質学会"}]}, "item_2_source_id_10": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1344-7882", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yajima, Kensuke"}], "nameIdentifiers": [{"nameIdentifier": "2809", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Onodera, Shuichi"}], "nameIdentifiers": [{"nameIdentifier": "2810", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeda, Yasuyuki"}], "nameIdentifiers": [{"nameIdentifier": "2811", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shiomi, Norio"}], "nameIdentifiers": [{"nameIdentifier": "2812", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-09-14"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "S-14_Onodera.pdf", "filesize": [{"value": "444.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 444400.0, "url": {"label": "S-14_Onodera.pdf", "url": "https://rakuno.repo.nii.ac.jp/record/1256/files/S-14_Onodera.pdf"}, "version_id": "666f8be1-9040-479f-8475-ac44dfb418dd"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Improved radical scavenging activity of β-lactoglobulin-xylobiose modified by the Maillard reaction", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Improved radical scavenging activity of β-lactoglobulin-xylobiose modified by the Maillard reaction"}]}, "item_type_id": "2", "owner": "3", "path": ["19"], "permalink_uri": "http://hdl.handle.net/10659/1396", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-03-11"}, "publish_date": "2010-03-11", "publish_status": "0", "recid": "1256", "relation": {}, "relation_version_is_last": true, "title": ["Improved radical scavenging activity of β-lactoglobulin-xylobiose modified by the Maillard reaction"], "weko_shared_id": -1}
Improved radical scavenging activity of β-lactoglobulin-xylobiose modified by the Maillard reaction
http://hdl.handle.net/10659/1396
http://hdl.handle.net/10659/139688879388-cc2b-45f1-ad07-a9d823c7d06b
名前 / ファイル | ライセンス | アクション |
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S-14_Onodera.pdf (444.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-03-11 | |||||
タイトル | ||||||
タイトル | Improved radical scavenging activity of β-lactoglobulin-xylobiose modified by the Maillard reaction | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yajima, Kensuke
× Yajima, Kensuke× Onodera, Shuichi× Takeda, Yasuyuki× Shiomi, Norio |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | β-Lactoglobulin (β-LG) was modified and conjugated to xylobiose using the Maillard reaction. The antioxidant activity of the Maillard reaction product, β-LG-xylobiose, was measured in vitro and compared to that of conjugated β-LG-lactose. The reaction for 7 days led to conjugated β-LG-xylobiose with a relative molecular mass ranging between 19 and 22 kDa based on SDS-PAGE analysis. It is confirmed that xylobiose bound to β-LG by gas-liquid chromatography. One milligram of conjugated β-LG-xylobiose contains 108 μg of xylobiose, while the available ε-amino group content decreased to 40% after the Maillard reaction. Conjugated β-LG-xylobiose had a higher radical scavenging activity than free β-LG. As the modification by xylobiose had a higher efficiency than the modification by lactose, it is found that xylobiose is a useful oligosaccharide for protein modification. Furthermore, the radical scavenging activity of β-LG was improved by modification with xylobiose. メイラード反応を利用してβ-lactoglobulin (β-LG)に,xylobioseを導入した.得られたβ-LG-xylobioseのin vitroにおける抗酸化活性をβ-LG-lactoseと比較検討した.7日間の反応を行ったところ,β-LG-xylobioseの分子量はSDS-PAGE分析により19-22 kDaと推定され,MALDI TOF-MS分析から約21 kDaを中心に多く分布していることがわかった.ガスクロマトグラフィー分析を行ったところ,xylobioseがβ-LGに結合していることが確認された.また,β-LG-xylobiose 1 mg当たり108 μgのxylobioseが結合したと算出され,遊離ε-アミノ基は約40%に減少したと算出された.DPPHを用いたラジカル捕捉能試験の結果より,β-LG-xylobioseはβ-LGよりも高いラジカル捕捉能を有していることが明らかになり,β-LG-lactoseのラジカル捕捉能よりも高かった.この結果より,xylobioseはタンパク質の糖化修飾における修飾糖として適したオリゴ糖と考えられる.以上の結果より,メイラード反応を利用したxylobiose糖化修飾によってβ-LGのラジカル捕捉能が向上することが確認された. | |||||
書誌情報 |
Journal of Applied Glycoscience 巻 54, 号 3, p. 165-168, 発行日 2007-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1344-7882 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | メイラード反応を利用したキシロビオース糖化β-Lactoglobulinのラジカル捕捉能の向上 | |||||
出版者 | ||||||
出版者 | 日本応用糖質学会 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |