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Detecting endotoxin activity in bovine serum using an automated testing system
http://hdl.handle.net/10659/5002
http://hdl.handle.net/10659/50020740330f-8625-4f54-bdd5-1d64e3e5a28c
名前 / ファイル | ライセンス | アクション |
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S-2016-198_suzuki.pdf (568.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-06-09 | |||||
タイトル | ||||||
タイトル | Detecting endotoxin activity in bovine serum using an automated testing system | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cattle | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | endotoxin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | limulus amebocyte lysate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | portable test system | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | serum | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
鈴木, 一由
× 鈴木, 一由× SHIMAMORI, Toshio× 佐藤, 綾乃× TSUKANO, Kenji× TSUCHIYA, Masakazu× LAKRITZ, Jeffrey |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The aim of the present study was to compare the ability of the commercially available portable test system (PTS^<TM>) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS^<TM> was not significantly different from that of the traditional LAL-based assays. The results using PTS^<TM> correlated with those using KT (r^2=0.963, P<0.001) or KC assays (r^2=0.982, P<0.001). Based on these findings, the PTS^<TM> could be applied as a simplified system to assess endotoxin activity in bovine serum. | |||||
書誌情報 |
The Journal of Veterinary Medical Science 巻 77, 号 8, p. 977-979, 発行日 2015-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-7250 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1292/jvms.14-0545 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 日本獣医学会 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |