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Expression of 5-HT_\u003c2A\u003e and 5-HT_7 receptors was examined by molecular biological study. 5-HT-containing cells were observed immunohistochemically. In the spontaneously contracting uterine circular muscle layers, 5-HT caused inhibition of contractile activity. SB269970 (5-HT_7 receptor antagonist, 10 nM) shifted the concentration-inhibition curve of 5-HT to the right, but higher concentrations of SB269970 (100 nM-1 μM) changed the monophasic curve to a biphasic curve (mixture of excitatory and inhibitory responses to 5-HT). Addition of ketanserin (5-HT_2 receptor antagonist, 10 nM-1 μM) decreased the excitatory effects of 5-HT. 5-HT was less effective in inhibiting the spontaneous contraction in the longitudinal muscles. Ketanserin enhanced the inhibitory responses and SB269970 reversed the inhibitory responses to excitatory responses. 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Excitatory and inhibitory 5-hydroxytryptamine (5-HT) receptors expressed in the isolated porcine uterine muscles
http://hdl.handle.net/10659/1791
http://hdl.handle.net/10659/179180754215-0e26-4489-aca9-f295b643e979
名前 / ファイル | ライセンス | アクション |
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S-518_Kitazawa.pdf (1.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-03-31 | |||||
タイトル | ||||||
タイトル | Excitatory and inhibitory 5-hydroxytryptamine (5-HT) receptors expressed in the isolated porcine uterine muscles | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | porcine uterus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 5-HT_<2A> receptor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 5-HT_7 receptor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 5-HT-immunoreactive cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | uterine contractility | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakamura, Tatsuro
× Nakamura, Tatsuro× Kitazawa, Takio× Cao, Jinshan× Iwamoto, Takuya× Teraoka, Hiroki× Kadota, Koichi× Taneike, Tetsuro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 5-Hydroxytryptamine (5-HT) receptors mediating excitatory and inhibitory actions of 5-HT on contractility of uterine strips from non-pregnant pigs were characterized. Expression of 5-HT_<2A> and 5-HT_7 receptors was examined by molecular biological study. 5-HT-containing cells were observed immunohistochemically. In the spontaneously contracting uterine circular muscle layers, 5-HT caused inhibition of contractile activity. SB269970 (5-HT_7 receptor antagonist, 10 nM) shifted the concentration-inhibition curve of 5-HT to the right, but higher concentrations of SB269970 (100 nM-1 μM) changed the monophasic curve to a biphasic curve (mixture of excitatory and inhibitory responses to 5-HT). Addition of ketanserin (5-HT_2 receptor antagonist, 10 nM-1 μM) decreased the excitatory effects of 5-HT. 5-HT was less effective in inhibiting the spontaneous contraction in the longitudinal muscles. Ketanserin enhanced the inhibitory responses and SB269970 reversed the inhibitory responses to excitatory responses. In the presence of SB269970, α-methyl-5-HT was equipotent to 5-HT in increasing contractility of longitudinal muscle and ketanserin competitively inhibited the responses to α-methyl-5-HT (pK_d = 8.78). Muscle layer-dependent expression of both 5-HT_<2A> receptor and 5-HT_7 receptor mRNAs in the porcine uterine muscle layers was demonstrated by RT-PCR and real-time PCR. 5-HT immunoreactivity was detected only in uterine gland cells, which were localized near the uterine circular muscle layers. In the longitudinal and circular muscle layers with endometrium, compounds 48/80 and ketanserin did not change the spontaneous contractility, but SB269970 significantly increased the contractile activity of the circular muscle. In conclusion, excitatory 5-HT_<2A> and inhibitory 5-HT_7 are present in the uterus of non-pregnant pigs. Endogenous 5-HT containing cells are mainly present in uterine glands of the pig. The possible roles of 5-HT and its receptors in regulation of porcine uterine spontaneous contractility are discussed. | |||||
書誌情報 |
European journal of pharmacology 巻 600, 号 1-3, p. 123-129, 発行日 2008-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0014-2999 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ejphar.2008.10.019 | |||||
権利(URI) | ||||||
権利情報 | http://dx.doi.org/10.1016/j.ejphar.2008.10.019 | http://dx.doi.org/10.1016/j.ejphar.2008.10.019 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |