WEKO3
アイテム
{"_buckets": {"deposit": "435535ef-2f88-495d-a53b-ddb355bedf51"}, "_deposit": {"created_by": 3, "id": "2202", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "2202"}, "status": "published"}, "_oai": {"id": "oai:rakuno.repo.nii.ac.jp:00002202", "sets": ["29"]}, "author_link": ["5723", "5728", "5726", "5724", "5729", "3287", "5727", "5725", "5722", "5730"], "item_2_biblio_info_9": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "115", "bibliographicPageStart": "111", "bibliographicVolumeNumber": "69", "bibliographic_titles": [{"bibliographic_title": "The Journal of Veterinary Medical Science"}]}]}, "item_2_description_43": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_2_description_6": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Cellular drug resistance to antineoplastic drugs is often due to the presence of a drug efflux pump that reduces intracellular drug accumulation and chemosensitivity. P-glycoprotein (P-gp), which is encoded by the MDR1 gene, is considered to function as an ATP-driven membrane drug efflux pump and appears to play an important role in tumor cell resistance. In the present report, we assessed the expression of MDR1 by RT-PCR in three canine mast cell tumor cell lines, TiMC, CoMS and LuMC, originating from a cutaneous tumor, an oral-mucosal tumor and a gastrointestinal tumor, respectively. P-gp expression was also examined by Western blot analysis, while the functional activity of P-gp was assessed by flowcytometric analysis of intracellular rhodamine-123 (Rhd-123) uptake. The results revealed that MDR1 gene and P-gp were both expressed in CoMS and LuMC cells, whereas neither was present in TiMC cells. In CoMS and LuMC cells, intracellular uptake of Rhd-123 increased in the presence of verapamil, a functional modulator of P-gp. In contrast, TiMC cells did not show any changes in the intracellular accumulation of Rhd-123 after the verapamil addition. These findings suggest that the expressions of MDR1 gene and P-gp probably contribute to cellular drug resistance in canine mast cell tumors.", "subitem_description_type": "Abstract"}]}, "item_2_publisher_36": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本獣医学会"}]}, "item_2_source_id_10": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0916-7250", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "NAKAICHI, Munekazu"}], "nameIdentifiers": [{"nameIdentifier": "5722", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TAKESHITA, Yoko"}], "nameIdentifiers": [{"nameIdentifier": "5723", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OKUDA, Masaru"}], "nameIdentifiers": [{"nameIdentifier": "5724", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NAKAMOTO, Yuya"}], "nameIdentifiers": [{"nameIdentifier": "5725", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ITAMOTO, Kazuhito"}], "nameIdentifiers": [{"nameIdentifier": "5726", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "UNE, Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "5727", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SASAKI, Nobuo"}], "nameIdentifiers": [{"nameIdentifier": "5728", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KADOSAWA, Tsuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "3287", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1017c99eddb77f9e0c0475b30845413d", "nameIdentifierScheme": "教員総覧", "nameIdentifierURI": "http://souran.rakuno.ac.jp/profile/ja.1017c99eddb77f9e0c0475b30845413d.html"}]}, {"creatorNames": [{"creatorName": "TAKAHASHI, Tomoko"}], "nameIdentifiers": [{"nameIdentifier": "5729", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TAURA, Yasuho"}], "nameIdentifiers": [{"nameIdentifier": "5730", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-09-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "S-172_Kadosawa.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "S-172_Kadosawa.pdf", "url": "https://rakuno.repo.nii.ac.jp/record/2202/files/S-172_Kadosawa.pdf"}, "version_id": "2e20b385-3688-47f8-822d-8b8cf579a0d1"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines"}]}, "item_type_id": "2", "owner": "3", "path": ["29"], "permalink_uri": "http://hdl.handle.net/10659/1429", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-03-11"}, "publish_date": "2010-03-11", "publish_status": "0", "recid": "2202", "relation": {}, "relation_version_is_last": true, "title": ["Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines"], "weko_shared_id": -1}
Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines
http://hdl.handle.net/10659/1429
http://hdl.handle.net/10659/1429cbdca490-0bb7-4156-af48-30c0e910d74b
名前 / ファイル | ライセンス | アクション |
---|---|---|
S-172_Kadosawa.pdf (1.1 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-03-11 | |||||
タイトル | ||||||
タイトル | Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
NAKAICHI, Munekazu
× NAKAICHI, Munekazu× TAKESHITA, Yoko× OKUDA, Masaru× NAKAMOTO, Yuya× ITAMOTO, Kazuhito× UNE, Satoshi× SASAKI, Nobuo× KADOSAWA, Tsuyoshi× TAKAHASHI, Tomoko× TAURA, Yasuho |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cellular drug resistance to antineoplastic drugs is often due to the presence of a drug efflux pump that reduces intracellular drug accumulation and chemosensitivity. P-glycoprotein (P-gp), which is encoded by the MDR1 gene, is considered to function as an ATP-driven membrane drug efflux pump and appears to play an important role in tumor cell resistance. In the present report, we assessed the expression of MDR1 by RT-PCR in three canine mast cell tumor cell lines, TiMC, CoMS and LuMC, originating from a cutaneous tumor, an oral-mucosal tumor and a gastrointestinal tumor, respectively. P-gp expression was also examined by Western blot analysis, while the functional activity of P-gp was assessed by flowcytometric analysis of intracellular rhodamine-123 (Rhd-123) uptake. The results revealed that MDR1 gene and P-gp were both expressed in CoMS and LuMC cells, whereas neither was present in TiMC cells. In CoMS and LuMC cells, intracellular uptake of Rhd-123 increased in the presence of verapamil, a functional modulator of P-gp. In contrast, TiMC cells did not show any changes in the intracellular accumulation of Rhd-123 after the verapamil addition. These findings suggest that the expressions of MDR1 gene and P-gp probably contribute to cellular drug resistance in canine mast cell tumors. | |||||
書誌情報 |
The Journal of Veterinary Medical Science 巻 69, 号 2, p. 111-115, 発行日 2007-02 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-7250 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 日本獣医学会 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |