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Fluoroquinolone resistance mechanisms in an Escherichia coli isolate, HUE1, without quinolone resistance-determining region mutations
http://hdl.handle.net/10659/3848
http://hdl.handle.net/10659/38481b686b3b-6693-4ab8-a9a1-251b10595c43
名前 / ファイル | ライセンス | アクション |
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S-2014-154_tamura.pdf (1.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-05-12 | |||||
タイトル | ||||||
タイトル | Fluoroquinolone resistance mechanisms in an Escherichia coli isolate, HUE1, without quinolone resistance-determining region mutations | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | AcrAB | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | efflux pump | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Escherichia coli | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fluoroquinolone resistance | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | oqxAB | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | qnrS | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sato, Toyotaka
× Sato, Toyotaka× Yokota, Shin-ichi× Uchida, Ikuo× Okubo, Torahiko× Usui, Masaru× Kusumoto, Masahiro× Akiba, Masato× Fujii, Nobuhiro× Tamura, Yutaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Fluoroquinolone resistance can cause major clinical problems. Here, we investigated fluoroquinolone resistance mechanisms in a clinical Escherichia coli isolate, HUE1, which had no mutations quinolone resistance-determining regions (QRDRs) of DNA gyrase and topoisomerase IV. HUE1 demonstrated MICs that exceeded the breakpoints for ciprofloxacin, levofloxacin, and norfloxacin. HUE1 harbored oqxAB and qnrS1 on distinct plasmids. In addition, it exhibited lower intracellular ciprofloxacin concentrations and higher mRNA expression levels of efflux pumps and their global activators than did reference strains. The genes encoding AcrR (local AcrAB repressor) and MarR (MarA repressor) were disrupted by insertion of the transposon IS3-IS629 and a frameshift mutation, respectively. A series of mutants derived from HUE1 were obtained by plasmid curing and gene knockout using homologous recombination. Compared to the MICs of the parent strain HUE1, the fluoroquinolone MICs of these mutants indicated that qnrS1, oqxAB, acrAB, acrF, acrD, mdtK, mdfA, and tolC contributed to the reduced susceptibility to fluoroquinolone in HUE1. Therefore, fluoroquinolone resistance in HUE1 is caused by concomitant acquisition of QnrS1 and OqxAB and overexpression of AcrAB-TolC and other chromosome-encoded efflux pumps. Thus, we have demonstrated that QRDR mutations are not absolutely necessary for acquiring fluoroquinolone resistance in E. coli. | |||||
書誌情報 |
Frontiers in Microbiology 巻 4, p. 125-1-125-12, 発行日 2013-05 |
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DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3389/fmicb.2013.00125 | |||||
権利 | ||||||
権利情報 | © 2013 Sato, Yokota, Uchida, Okubo, Usui, Kusumoto, Akiba, Fujii and Tamura. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |