WEKO3
アイテム
{"_buckets": {"deposit": "497c0938-ce83-4f1e-adfe-9ea920ef7875"}, "_deposit": {"created_by": 3, "id": "2572", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "2572"}, "status": "published"}, "_oai": {"id": "oai:rakuno.repo.nii.ac.jp:00002572", "sets": ["29"]}, "author_link": ["7731", "7738", "7737", "7741", "1839", "7740", "7735", "7736", "7732", "7733", "7739", "5088", "7734"], "item_2_alternative_title_22": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Parasite Identification Based on the Sequences of COX1 and ITS Obtained from Fecal Eggs in an Asian Tapir (Tapirus indicus)"}]}, "item_2_biblio_info_9": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "37", "bibliographicPageStart": "31", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{"bibliographic_title": "日本野生動物医学会誌"}, {"bibliographic_title": "Japanese journal of zoo and wildlife medicine", "bibliographic_titleLang": "en"}]}]}, "item_2_description_43": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_2_description_6": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "On November 13, 2000, an Asian tapir (Tapirus indicus) was transferred from the Melaka Zoo in Malaysia to the Sapporo Maruyama Zoo in Japan. During quarantine at the Sapporo Maruyama Zoo, nematode and cestode eggs were observed and disinfestation was conducted using Praziquantel. Segments were found in feces, but it was impossible to identify the cestode species morphologically because all were gravid segments filled with eggs. DNA extraction was conducted from the parasite eggs in the feces and from the segments. Sequences of cytochrome c oxidase subunit 1 (COX1) in mitochondrial DNA and internal transcribed spacer (ITS) were ascertained. The results of a homology search showed that the sequences of ITS obtained from both the parasite eggs and the segments were similar to the sequence of a fungus. Two kinds of COX 1 sequence were obtained from the parasite eggs; one was similar to Ancylostoma, while the other, a sequence that was the same as that obtained from the segments, was related to Paranoplocephala. The results of the present study indicated that species identification might be possible based on sequences obtained from parasite eggs in feces. Furthermore, the method might be useful for diagnosis of parasite infections in wild species with only scarce information about parasitic helminth fauna. マラッカ動物園(マレーシア)より札幌市円山動物園に搬入された一頭のマレーバク(Tapirus indicus)の糞便検査を行ったところ,線虫卵と多数の条虫卵を検出した。プラジクアンテル投与後,糞中より条虫片節が採取されたが老熟片節であった。そこで,糞便中の虫卵および駆虫後排泄された片節からDNAを抽出し,ミトコンドリアのcytochrome c oxidase subunit 1(COX1)遺伝子および核ゲノム中に存在するInternal transcrbed spacer領域(ITS)を対象にPCRを行った。このとき,条虫類と線虫類に共通して使用できるプライマーを用いた。その後PCR産物の塩基配列を決定し,BLASTによるホモロジー検索を行った。その結果,ITS領域用プライマーによって得られた,虫卵および片節由来のPCR産物はすべて真菌類ITSの配列と類似していた。一方,COX1用プライマーによって得られた虫卵由来のPCR産物からは2種類の配列が得られ,一つは裸頭条虫科(Anoplocephalidae)のParanoploaephala属に,もう一つは鈎虫科(Ancylostomidae)のAncylostoma属に近縁の配列であった。このことから本研究において応用された方法は,寄生嬬虫相の情報が少ない野生動物の寄生嬬虫症診断法として有用である可能性が示唆された。", "subitem_description_type": "Abstract"}]}, "item_2_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "7736", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "OTSUKA, Hiroko"}]}, {"nameIdentifiers": [{"nameIdentifier": "7737", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "ONUMA, Manabu"}]}, {"nameIdentifiers": [{"nameIdentifier": "7738", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "FUKUMOTO, Shin-ichiro"}]}, {"nameIdentifiers": [{"nameIdentifier": "7739", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "MUKAI, Takeshi"}]}, {"nameIdentifiers": [{"nameIdentifier": "7740", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "SHIROZU, Aya"}]}, {"nameIdentifiers": [{"nameIdentifier": "7741", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "CHIBA, Tsukasa"}]}, {"affiliations": [{"affiliationNames": [{"affiliationName": "", "lang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "", "nameIdentifierScheme": "ISNI", "nameIdentifierURI": "http://www.isni.org/isni/"}]}], "familyNames": [{"familyName": "浅川", "familyNameLang": "ja"}, {"familyName": "ASAKAWA", "familyNameLang": "en"}], "givenNames": [{"givenName": "満彦", "givenNameLang": "ja"}, {"givenName": "Mitsuhiko", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1839", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9416", "nameIdentifierScheme": "教員総覧", "nameIdentifierURI": "https://www.rakuno.ac.jp/archives/teacher/9416.html"}], "names": [{"name": "浅川, 満彦", "nameLang": "ja"}, {"name": "ASAKAWA, Mitsuhiko", "nameLang": "en"}]}]}, "item_2_publisher_36": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本野生動物医学会"}]}, "item_2_source_id_10": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1342-6133", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "大塚, 浩子"}], "nameIdentifiers": [{"nameIdentifier": "7731", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "大沼, 学"}], "nameIdentifiers": [{"nameIdentifier": "7732", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "福本, 真一郎"}], "nameIdentifiers": [{"nameIdentifier": "5088", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "cb22d3fb8f00ce5260e1a3bbf8166b98", "nameIdentifierScheme": "教員総覧", "nameIdentifierURI": "http://souran.rakuno.ac.jp/profile/ja.cb22d3fb8f00ce5260e1a3bbf8166b98.html"}]}, {"creatorNames": [{"creatorName": "向井, 猛"}], "nameIdentifiers": [{"nameIdentifier": "7733", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "白水, 彩"}], "nameIdentifiers": [{"nameIdentifier": "7734", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "千葉, 司"}], "nameIdentifiers": [{"nameIdentifier": "7735", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "浅川, 満彦", "creatorNameLang": "ja"}, {"creatorName": "ASAKAWA, Mitsuhiko", "creatorNameLang": "en"}], "familyNames": [{"familyName": "浅川", "familyNameLang": "ja"}, {"familyName": "ASAKAWA", "familyNameLang": "en"}], "givenNames": [{"givenName": "満彦", "givenNameLang": "ja"}, {"givenName": "Mitsuhiko", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1839", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9416", "nameIdentifierScheme": "教員総覧", "nameIdentifierURI": "https://www.rakuno.ac.jp/archives/teacher/9416.html"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-09-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "S-2015-493_asakawa.pdf", "filesize": [{"value": "695.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 695800.0, "url": {"label": "S-2015-493_asakawa.pdf", "url": "https://rakuno.repo.nii.ac.jp/record/2572/files/S-2015-493_asakawa.pdf"}, "version_id": "31cc79c1-35c8-47ea-9910-b8500a123f48"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Asian tapir", "subitem_subject_scheme": "Other"}, {"subitem_subject": "feces", "subitem_subject_scheme": "Other"}, {"subitem_subject": "helminth egg", "subitem_subject_scheme": "Other"}, {"subitem_subject": "COX1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ITS", "subitem_subject_scheme": "Other"}, {"subitem_subject": "マレーバク", "subitem_subject_scheme": "Other"}, {"subitem_subject": "糞便", "subitem_subject_scheme": "Other"}, {"subitem_subject": "嬬虫卵", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "マレーバク(Tapirus indicus)糞便中に認められた虫卵のCOX1およびITS領域塩基配列を指標とした寄生蠕虫類同定の試み", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "マレーバク(Tapirus indicus)糞便中に認められた虫卵のCOX1およびITS領域塩基配列を指標とした寄生蠕虫類同定の試み"}]}, "item_type_id": "2", "owner": "3", "path": ["29"], "permalink_uri": "http://hdl.handle.net/10659/4752", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-05-23"}, "publish_date": "2016-05-23", "publish_status": "0", "recid": "2572", "relation": {}, "relation_version_is_last": true, "title": ["マレーバク(Tapirus indicus)糞便中に認められた虫卵のCOX1およびITS領域塩基配列を指標とした寄生蠕虫類同定の試み"], "weko_shared_id": -1}
マレーバク(Tapirus indicus)糞便中に認められた虫卵のCOX1およびITS領域塩基配列を指標とした寄生蠕虫類同定の試み
http://hdl.handle.net/10659/4752
http://hdl.handle.net/10659/475210c6e665-0543-4c92-b7f9-966d4f4aec31
名前 / ファイル | ライセンス | アクション |
---|---|---|
S-2015-493_asakawa.pdf (695.8 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2016-05-23 | |||||
タイトル | ||||||
タイトル | マレーバク(Tapirus indicus)糞便中に認められた虫卵のCOX1およびITS領域塩基配列を指標とした寄生蠕虫類同定の試み | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Asian tapir | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | feces | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | helminth egg | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | COX1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ITS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | マレーバク | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 糞便 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 嬬虫卵 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
大塚, 浩子
× 大塚, 浩子× 大沼, 学× 福本, 真一郎× 向井, 猛× 白水, 彩× 千葉, 司× 浅川, 満彦 |
|||||
著者別名 | ||||||
姓名 | OTSUKA, Hiroko | |||||
著者別名 | ||||||
姓名 | ONUMA, Manabu | |||||
著者別名 | ||||||
姓名 | FUKUMOTO, Shin-ichiro | |||||
著者別名 | ||||||
姓名 | MUKAI, Takeshi | |||||
著者別名 | ||||||
姓名 | SHIROZU, Aya | |||||
著者別名 | ||||||
姓名 | CHIBA, Tsukasa | |||||
著者別名 | ||||||
姓名 | 浅川, 満彦 | |||||
言語 | ja | |||||
姓名 | ASAKAWA, Mitsuhiko | |||||
言語 | en | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | On November 13, 2000, an Asian tapir (Tapirus indicus) was transferred from the Melaka Zoo in Malaysia to the Sapporo Maruyama Zoo in Japan. During quarantine at the Sapporo Maruyama Zoo, nematode and cestode eggs were observed and disinfestation was conducted using Praziquantel. Segments were found in feces, but it was impossible to identify the cestode species morphologically because all were gravid segments filled with eggs. DNA extraction was conducted from the parasite eggs in the feces and from the segments. Sequences of cytochrome c oxidase subunit 1 (COX1) in mitochondrial DNA and internal transcribed spacer (ITS) were ascertained. The results of a homology search showed that the sequences of ITS obtained from both the parasite eggs and the segments were similar to the sequence of a fungus. Two kinds of COX 1 sequence were obtained from the parasite eggs; one was similar to Ancylostoma, while the other, a sequence that was the same as that obtained from the segments, was related to Paranoplocephala. The results of the present study indicated that species identification might be possible based on sequences obtained from parasite eggs in feces. Furthermore, the method might be useful for diagnosis of parasite infections in wild species with only scarce information about parasitic helminth fauna. マラッカ動物園(マレーシア)より札幌市円山動物園に搬入された一頭のマレーバク(Tapirus indicus)の糞便検査を行ったところ,線虫卵と多数の条虫卵を検出した。プラジクアンテル投与後,糞中より条虫片節が採取されたが老熟片節であった。そこで,糞便中の虫卵および駆虫後排泄された片節からDNAを抽出し,ミトコンドリアのcytochrome c oxidase subunit 1(COX1)遺伝子および核ゲノム中に存在するInternal transcrbed spacer領域(ITS)を対象にPCRを行った。このとき,条虫類と線虫類に共通して使用できるプライマーを用いた。その後PCR産物の塩基配列を決定し,BLASTによるホモロジー検索を行った。その結果,ITS領域用プライマーによって得られた,虫卵および片節由来のPCR産物はすべて真菌類ITSの配列と類似していた。一方,COX1用プライマーによって得られた虫卵由来のPCR産物からは2種類の配列が得られ,一つは裸頭条虫科(Anoplocephalidae)のParanoploaephala属に,もう一つは鈎虫科(Ancylostomidae)のAncylostoma属に近縁の配列であった。このことから本研究において応用された方法は,寄生嬬虫相の情報が少ない野生動物の寄生嬬虫症診断法として有用である可能性が示唆された。 | |||||
書誌情報 |
日本野生動物医学会誌 en : Japanese journal of zoo and wildlife medicine 巻 9, 号 1, p. 31-37, 発行日 2004-03 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1342-6133 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | Parasite Identification Based on the Sequences of COX1 and ITS Obtained from Fecal Eggs in an Asian Tapir (Tapirus indicus) | |||||
出版者 | ||||||
出版者 | 日本野生動物医学会 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |