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Comparison of Cryotop and micro volume air cooling methods for cryopreservation of bovine matured oocytes and blastocysts
http://hdl.handle.net/10659/4934
http://hdl.handle.net/10659/4934808eb3a2-e807-4438-a6fb-a414e2b1693a
名前 / ファイル | ライセンス | アクション |
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S-2016-026_imai.pdf (673.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-06-09 | |||||
タイトル | ||||||
タイトル | Comparison of Cryotop and micro volume air cooling methods for cryopreservation of bovine matured oocytes and blastocysts | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Blastocysts | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cryodevice | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cryotop | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Micro volume air cooling | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Oocytes | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
PUNYAWAI, Kanchana
× PUNYAWAI, Kanchana× ANAKKUL, Nitira× SRIRATTANA, Kanokwan× AIKAWA, Yoshio× SANGSRITAVONG, Siwat× NAGAI, Takashi× 今井, 敬× PARNPAI, Rangsun |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | This study was designed to compare the efficiency of the Cryotop method and that of two methods that employ a micro volume air cooling (MVAC) device by analyzing the survival and development of bovine oocytes and blastocysts vitrified using each method. In experiment I, in vitro-matured (IVM) oocytes were vitrified using an MVAC device without direct contact with liquid nitrogen (LN_2; MVAC group) or directly plunged into LN_2 (MVAC in LN_2 group). A third group of IVM oocytes was vitrified using a Cryotop device (Cryotop group). After warming, vitrified oocytes were fertilized in vitro. There were no significant differences in cleavage and blastocyst formation rates among the three vitrified groups, with the rates ranging from 53.1% to 56.6% and 20.0% to 25.5%, respectively; however, the rates were significantly lower (P < 0.05) than those of the fresh control group (89.3% and 43.3%, respectively) and the solution control group (87.3% and 42.0%, respectively). In experiment II, in vitro-produced (IVP) expanded blastocysts were vitrified using the MVAC, MVAC in LN_2 and Cryotop methods, warmed and cultured for survival analysis and then compared with the solution control group. The rate of development of vitrified-warmed expanded blastocysts to the hatched blastocyst stage after 24 h of culture was lower in the MVAC in LN_2 group than in the solution control group; however, after 48–72 h of culture, the rates did not significantly differ between the groups. These results indicate that the MVAC method without direct LN_2 contact is as effective as the standard Cryotop method for vitrification of bovine IVM oocytes and IVP expanded blastocysts. | |||||
書誌情報 |
Journal of Reproduction and Development 巻 61, 号 5, p. 431-437, 発行日 2015-10 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-8818 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1262/jrd.2014-163 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 日本繁殖生物学会 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |