WEKO3
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Cytoskeletal and mitochondrial properties of bovine oocytes obtained by Ovum Pick-Up: the effects of follicle stimulation and in vitro maturation
http://hdl.handle.net/10659/4931
http://hdl.handle.net/10659/4931fefb53b7-23dd-46b0-a11f-5f12d7446a88
名前 / ファイル | ライセンス | アクション |
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S-2016-004_imai.pdf (786.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-06-09 | |||||
タイトル | ||||||
タイトル | Cytoskeletal and mitochondrial properties of bovine oocytes obtained by Ovum Pick-Up: the effects of follicle stimulation and in vitro maturation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bovine oocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | in vitro maturation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | in vivo maturation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cytoskeleton | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mitochondria | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Somfai, Tamás
× Somfai, Tamás× Matoba, Satoko× Inaba, Yasushi× Nakai, Michiko× Imai, Kei× Nagai, Takashi× Geshi, Masaya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Follicle stimulation by follicular stimulating hormone (FSH) is known to improve developmental competence of bovine oocytes obtained by Ovum Pick-Up (OPU); however, the exact factors in oocytes affected by this treatment have remained unclear. We compared in vitro matured (IVM) oocytes obtained at the immature stage from cows by OPU either without or with stimulation with FSH (non-stimulated and stimulated OPU, respectively) to those obtained by superstimulation and in vivo maturation in terms of cytoskeleton morphology, mitochondrial distribution, intracellular adenosine triphosphate (ATP) content and H_2O_2 levels at the metaphase-II stage and intracellular Ca^<2+> levels after in vitro fertilization (IVF). Confocal microscopy after immunostaining revealed reduced size of the meiotic spindle, associated with increased tendencies of microfilament degradation and insufficient mitochondrial re-distribution in non-stimulated OPU-derived IVM oocytes compared with those collected by stimulated OPU, which in turn resembled in vivo matured oocytes. However, there was no difference in mitochondrial functions between oocytes obtained by stimulated or non-stimulated OPU in terms of ATP content, cytoplasmic H_2O_2 levels, base Ca^<2+> levels and the frequencies and amplitudes of Ca^<2+> oscillations after IVF. Larger size of metaphase spindles in oocytes obtained by stimulated OPU may reflect and potentially contribute to their high developmental competence. | |||||
書誌情報 |
Animal Science Journal 巻 86, 号 12, p. 970-980, 発行日 2015-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1740-0929 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/asj.12387 | |||||
権利 | ||||||
権利情報 | This is the peer reviewed version of the following article: John Wiley and Sons, Somfai, Tamás ; Matoba, Satoko ; Inaba, Yasushi ; Nakai, Michiko ; Imai, Kei ; Nagai, Takashi ; Geshi, Masaya, Animal Science Journal, 86(12), p970-980, which has been published in final form at http://dx.doi.org/10.1111/asj.12387. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
出版者 | ||||||
出版者 | John Wiley and Sons | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |