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Ring-Mesh Model of Proteoglycan Glycosaminoglycan Chains in Tendon based on Three-dimensional Reconstruction by Focused Ion Beam Scanning Electron Microscopy
http://hdl.handle.net/10659/00005387
http://hdl.handle.net/10659/00005387e23805cf-d566-44dd-92cd-8a12b189e404
名前 / ファイル | ライセンス | アクション |
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R-2017-56_Takehana.pdf (1.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-04-12 | |||||
タイトル | ||||||
タイトル | Ring-Mesh Model of Proteoglycan Glycosaminoglycan Chains in Tendon based on Three-dimensional Reconstruction by Focused Ion Beam Scanning Electron Microscopy | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Watanabe, Takafumi
× Watanabe, Takafumi× Kametani, Kiyokazu× Koyama, Yoh-ichi× Suzuki, Daisuke× Imamura, Yasutada× Takehana, Kazushige× Hiramatsu, Kohzy |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Tendons are composed of collagen fibrils and proteoglycan predominantly consisting of decorin. Decorin is located on the d-band of collagen fibrils, and its glycosaminoglycan (GAG) chains have been observed between collagen fibrils with transmission electron microscopy. GAG chains have been proposed to interact with each other or with collagen fibrils, but its three-dimensional organization remains unclear. In this report, we used focused ion beam scanning electron microscopy to examine the three-dimensional organization of the GAG chain in the Achilles tendon of mature rats embedded in epoxy resin after staining with Cupromeronic blue, which specifically stains GAG chains. We used 250 serial back-scattered electron images of longitudinal sections with a 10-nm interval for reconstruction. Three-dimensional images revealed that GAG chains form a ring mesh-like structure with each ring surrounding a collagen fibril at the d-band and fusing with adjacent rings to form the planar network. This ring mesh model of GAG chains suggests that more than two GAG chains may interact with each other around collagen fibrils, which could provide new insights into the roles of GAG chains. | |||||
書誌情報 |
The Journal of Biological Chemistry 巻 291, 号 45, p. 23704-23708, 発行日 2016-11 |
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ISBN | ||||||
識別子タイプ | ISBN | |||||
関連識別子 | 1083-351X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.1074/jbc.M116.733857 | |||||
権利 | ||||||
権利情報 | This research was originally published in The Journal of Biological Chemistry. Takafumi Watanabe, Kiyokazu Kametani, Yoh-ichi Koyama, Daisuke Suzuki, Yasutada Imamura, Kazushige Takehana and Kohzy Hiramatsu. Ring-Mesh Model of Proteoglycan Glycosaminoglycan Chains in Tendon based on Three-dimensional Reconstruction by Focused Ion Beam Scanning Electron Microscopy. The Journal of Biological Chemistry. 2016; 291(45): 23704-23708. © the American Society for Biochemistry and Molecular Biology. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | American Society for Biochemistry and Molecular Biology | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |