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Suppressive Effects of the Site 1 Protease (S1P) Inhibitor, PF-429242, on Dengue Virus Propagation
http://hdl.handle.net/10659/00005441
http://hdl.handle.net/10659/00005441359649fe-25c1-4dd5-8da5-99cf6b479cea
名前 / ファイル | ライセンス | アクション |
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R-2017-226_Uchida.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-04-12 | |||||
タイトル | ||||||
タイトル | Suppressive Effects of the Site 1 Protease (S1P) Inhibitor, PF-429242, on Dengue Virus Propagation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | dengue virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antivirus drug | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PF-429242 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SREBPs | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | S1P | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
内田, 玲麻
× 内田, 玲麻× Urata, Shuzo× Ulanday, Gianne Eduard L.× Takamatsu, Yuki× Yasuda, Jiro× Morita, Kouichi× Hayasaka, Daisuke |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Dengue virus (DENV) infection causes one of the most widespread mosquito-borne diseases in the world. Despite the great need, effective vaccines and practical antiviral therapies are still under development. Intracellular lipid levels are regulated by sterol regulatory elements-binding proteins (SREBPs), which are activated by serine protease, site 1 protease (S1P). Small compound PF-429242 is known as a S1P inhibitor and the antivirus effects have been reported in some viruses. In this study, we examined the anti-DENV effects of PF-429242 using all four serotypes of DENV by several primate-derived cell lines. Moreover, emergence of drug-resistant DENV mutants was assessed by sequential passages with the drug. DENV dependency on intracellular lipids during their infection was also evaluated by adding extracellular lipids. The addition of PF-429242 showed suppression of viral propagation in all DENV serotypes. We showed that drug-resistant DENV mutants are unlikely to emerge after five times sequential passages through treatment with PF-429242. Although the levels of intracellular cholesterol and lipid droplets were reduced by PF-429242, viral propagations were not recovered by addition of exogenous cholesterol or fatty acids, indicating that the reduction of LD and cholesterol caused by PF-429242 treatment is not related to its mechanism of action against DENV propagation. Our results suggest that PF-429242 is a promising candidate for an anti-DENV agent. | |||||
書誌情報 |
Viruses 巻 8, 号 2, p. 46-1-46-12, 発行日 2016-02 |
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ISBN | ||||||
識別子タイプ | ISBN | |||||
関連識別子 | 1999-4915 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.3390/v8020046 | |||||
権利 | ||||||
権利情報 | © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license. | |||||
権利(URI) | ||||||
権利情報 | https://creativecommons.org/licenses/by/4.0/ | https://creativecommons.org/licenses/by/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | MDPI AG | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |