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自動rRNA遺伝子間多型解析(ARISA)によるキノコ種鑑別の検討とカキシメジによる食中毒疑い事例への適用の試み
http://hdl.handle.net/10659/00005450
http://hdl.handle.net/10659/0000545050ff6f7a-b8c1-4179-b3d2-93c64c1b83b6
名前 / ファイル | ライセンス | アクション |
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R-2017-249_Yamaguchi.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-04-12 | |||||
タイトル | ||||||
タイトル | 自動rRNA遺伝子間多型解析(ARISA)によるキノコ種鑑別の検討とカキシメジによる食中毒疑い事例への適用の試み | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA多型 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA polymorphism | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 品種鑑定 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | species identification | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | キノコ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mushroom | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 自然毒 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | natural toxin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 食中毒 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | food poisoning | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
菅原, 諒太
× 菅原, 諒太× 山田, さゆみ× 涂, 志豪× 菅原, 明子× 干場, 敏博× 栄坂, 貞夫× 山口, 昭弘 |
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著者別名 | ||||||
姓名 | Sugawara, Ryota | |||||
著者別名 | ||||||
姓名 | Yamada, Sayumi | |||||
著者別名 | ||||||
姓名 | Tu, Zhihao | |||||
著者別名 | ||||||
姓名 | Sugawara, Akiko | |||||
著者別名 | ||||||
姓名 | Hoshiba, Toshihiro | |||||
著者別名 | ||||||
姓名 | Eisaka, Sadao | |||||
著者別名 | ||||||
姓名 | Yamaguchi, Akihiro | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Automated rRNA intergenic spacer analysis (ARISA), a method of microbiome analysis, was evaluated for species identification of mushrooms based on the specific fragment sizes. We used 51 wild mushroom-fruiting bodies collected in the centre of Hokkaido and two cultivated mushrooms. Samples were hot-air-dried and DNA were extracted by a beads beating procedure. Sequencing analysis of portions of the rRNA gene (rDNA) provided 33 identifications of mushrooms by genus or species. The results of ARISA identification based on the combination of the fragment sizes corresponding to two inter spacer regions (ITS2 and ITS1) of rDNA within±0.1% accuracy showed that 27 out of the 33 species had specific fragment sizes differentiated from other species. The remaining 6 species formed 3 pairs that showed overlapping fragment sizes. In addition, within-species polymorphisms were observed as 1 bp differences among 32 samples of 13 species. ARISA was applied to investigate a case of suspected food poisoning in which the mushroom was thought to be a toxic Kakishimeji. The morphological identification of the mushroom was ambiguous since the remaining sample lacked a part of the fruiting body. Further, yeast colonies had grown on the surface of the fruiting body during storage. The ARISA fragment size of the mushroom showed 7 bp difference from that of the candidate toxic mushroom. Although ARISA could be a useful tools for estimation of mushroom species, especially in case where the fruiting bodies have deteriorated or been processed, further studies are necessary for reliable identification. For example, it may be necessary to adopt more informative genes which could provide clearer species-specific polymorphisms than the ITS regions. | |||||
書誌情報 |
食品衛生学雑誌 = Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 巻 57, 号 2, p. 37-45, 発行日 2016-04 |
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ISBN | ||||||
識別子タイプ | ISBN | |||||
関連識別子 | 0015-6426 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.3358/shokueishi.57.37 | |||||
権利 | ||||||
権利情報 | この論文は「食品衛生学雑誌」に掲載されたものであり、著作権のある(公社)日本食品衛生学会 の許諾を受けて公開しております。 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | Identification of Mushroom Species by Automated rRNA Intergenic Spacer Analysis(ARISA) and Its Application to a Suspected Case of Food Poisoning with Tricholoma ustale | |||||
出版者 | ||||||
出版者 | 日本食品衛生学会 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |