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Characterization of pertussis-like toxin from Salmonella spp. that catalyzes ADP-ribosylation of G proteins
http://hdl.handle.net/10659/00005916
http://hdl.handle.net/10659/000059165d26ae6d-1e57-44bb-9363-79e81cacc53c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
R-2018-164_uchida.pdf (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2019-08-08 | |||||
| タイトル | ||||||
| タイトル | Characterization of pertussis-like toxin from Salmonella spp. that catalyzes ADP-ribosylation of G proteins | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Tamamura, Yukino
× Tamamura, Yukino× Tanaka, Kiyoshi× Uchida, Ikuo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Salmonella Typhimurium definitive phage type (DT) 104 produces a pertussis-like toxin (ArtAB-DT104), which catalyzes ADP-ribosylation of pertussis toxin sensitive G proteins. However, the prevalence of ArtAB and its toxicity have not been established. We report here that, in addition to DT104, S. Worthington, and S. bongori, produce ArtAB homologs, designated ArtAB-SW and ArtAB-Sb, respectively. We purified and characterized these ArtAB toxins, which comprise a 27-kDa A subunit (ArtA) and 13.8-kDa pentameric B subunits (ArtB). While the sequence of the A subunit, which is ADP-ribosyltransferase, is similar to the A subunit sequences of other ArtABs, the B subunit of ArtAB-Sb is divergent compared to the B subunit sequences of other ArtABs. Intraperitoneal injection of purified ArtABs was fatal in mice; the 50% lethal doses of ArtAB-DT104 and ArtAB-SW were lower than that of ArtAB-Sb, suggesting that ArtB plays an influential role in the toxicity of ArtABs. ArtABs catalyzed ADP-ribosylation of G proteins in RAW 264.7 murine macrophage-like cells, and increased intracellular cyclic AMP levels. ArtAB-DT104 and ArtAB-SW, but not ArtAB-Sb, stimulated insulin secretion in mice; however, unlike Ptx, ArtABs did not induce leukocytosis. This disparity in biological activity may be explained by differences in ADP-ribosylation of target G proteins. | |||||
| 書誌情報 |
Scientific Reports 巻 7, p. 2653-1-2653-13, 発行日 2017-06 |
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| 識別子タイプ | ISBN | |||||
| 関連識別子 | 2045-2322 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://dx.doi.org/10.1038/s41598-017-02517-2 | |||||
| 権利 | ||||||
| 権利情報 | © The Author(s) 2017 | |||||
| 権利（URI） | ||||||
| 権利情報 | http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | Springer Nature Publishing AG | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
