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Characterization of the Lytic Capability of a LysK-Like Endolysin, Lys-phiSA012, Derived from a Polyvalent Staphylococcus aureus Bacteriophage
http://hdl.handle.net/10659/00006317
http://hdl.handle.net/10659/00006317878e2c73-d898-4853-9eb2-aa1c6516bb2a
名前 / ファイル | ライセンス | アクション |
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R-2019-75_iwano.pdf (3.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-02-26 | |||||
タイトル | ||||||
タイトル | Characterization of the Lytic Capability of a LysK-Like Endolysin, Lys-phiSA012, Derived from a Polyvalent Staphylococcus aureus Bacteriophage | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antibiotic resistant | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | multidrug resistant | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antimicrobial agent | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phage therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bacteriophage | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | endolysin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | staphylococci | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Staphylococcus aureus | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | antibiotic resistant | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | multidrug resistant | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | antimicrobial agent | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phage therapy | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | bacteriophage | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | endolysin | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | staphylococci | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Staphylococcus aureus | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
藤木, 純平
× 藤木, 純平× Nakamura, Tomohiro× Furusawa, Takaaki× Ohno, Hazuki× Takahashi, Hiromichi× Kitana, Junya× 臼井, 優× 樋口, 豪紀× Tanji, Yasunori× Tamura, Yutaka× 岩野, 英知 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Antibiotic-resistant bacteria (ARB) have spread widely and rapidly, with their increased occurrence corresponding with the increased use of antibiotics. Infections caused by Staphylococcus aureus have a considerable negative impact on human and livestock health. Bacteriophages and their peptidoglycan hydrolytic enzymes (endolysins) have received significant attention as novel approaches against ARB, including S. aureus. In the present study, we purified an endolysin, Lys-phiSA012, which harbors a cysteine/histidine-dependent amidohydrolase/peptidase (CHAP) domain, an amidase domain, and a SH3b cell wall binding domain, derived from a polyvalent S. aureus bacteriophage which we reported previously. We demonstrate that Lys-phiSA012 exhibits high lytic activity towards staphylococcal strains, including methicillin-resistant S. aureus (MRSA). Analysis of deletion mutants showed that only mutants possessing the CHAP and SH3b domains could lyse S. aureus, indicating that lytic activity of the CHAP domain depended on the SH3b domain. The presence of at least 1 mM Ca2+ and 100 µM Zn2+ enhanced the lytic activity of Lys-phiSA012 in a turbidity reduction assay. Furthermore, a minimum inhibitory concentration (MIC) assay showed that the addition of Lys-phiSA012 decreased the MIC of oxacillin. Our results suggest that endolysins are a promising approach for replacing current antimicrobial agents and may contribute to the proper use of antibiotics, leading to the reduction of ARB. | |||||
書誌情報 |
Pharmaceuticals 巻 11, 号 1, p. 25-1-25-19, 発行日 2018-02 |
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DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.3390/ph11010025 | |||||
権利 | ||||||
権利情報 | © 2018 by the authors. Licensee MDPI, Basel, Switzerland. | |||||
権利(URI) | ||||||
権利情報 | https://creativecommons.org/licenses/by/4.0/ | https://creativecommons.org/licenses/by/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | MDPI | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |