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Basement membrane-like structures containing NTH α1(IV) are formed around the endothelial cell network in a novel in vitro angiogenesis model
http://hdl.handle.net/10659/00006630
http://hdl.handle.net/10659/000066307063e2cb-a5a2-4653-bd74-2736dabf8b35
名前 / ファイル | ライセンス | アクション |
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R-2020-53_watanabe.pdf (8.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-11-18 | |||||
タイトル | ||||||
タイトル | Basement membrane-like structures containing NTH α1(IV) are formed around the endothelial cell network in a novel in vitro angiogenesis model | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | angiogenesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ascorbic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | basement membrane | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | coculture spheroid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nontriple helical polypeptide of type IV collagen α1 chain | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | angiogenesis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | ascorbic acid | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | basement membrane | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | coculture spheroid | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | nontriple helical polypeptide of type IV collagen α1 chain | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shin, Yongchol
× Shin, Yongchol× Moriya, Akane× Tohnishi, Yuta× 渡邉, 敬文× Imamura, Yasutada |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Angiogenesis is a process through which new blood vessels are formed by sprouting and elongating from existing blood vessels. Several methods have been used to replicate angiogenesis in vitro, including culturing vascular endothelial cells on Matrigel and coculturing with endothelial cells and fibroblasts. However, the angiogenesis elongation process has not been completely clarified in these models. We therefore propose a new in vitro model of angiogenesis, suitable for observing vascular elongation, by seeding a spheroid cocultured from endothelial cells and fibroblasts into a culture dish. In this model, endothelial cells formed tubular networks elongated from the spheroid with a lumen structure and were connected with tight junctions. A basement membrane (BM)-like structure was observed around the tubular network, similarly to blood vessels in vivo. These results suggested that blood vessel-like structure could be reconstituted in our model. Laminin and type IV collagen, main BM components, were highly localized around the network, along with nontriple helical form of type IV collagen α1-chain [NTH α1(IV)]. In an ascorbic acid-depleted condition, laminin and NTH α1(IV) were observed around the network but not the triple-helical form of type IV collagen and the network was unstable. These results suggest that laminin and NTH α1(IV) are involved in the formation of tubular network and type IV collagen is necessary to stabilize the network. | |||||
書誌情報 |
American Journal of Physiology Cell Physiology 巻 317, 号 2, p. 315-325, 発行日 2019-08 |
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ISBN | ||||||
識別子タイプ | ISBN | |||||
関連識別子 | 0363-6143 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1152/ajpcell.00353.2018 | |||||
権利 | ||||||
権利情報 | © 2019 the American Physiological Society | |||||
権利(URI) | ||||||
権利情報 | http://creativecommons.org/licenses/by/4.0/deed.en_US | http://creativecommons.org/licenses/by/4.0/deed.en_US | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | the American Physiological Society | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |