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Purification and Biochemical Characterization of Cysteine Protease from Baby Kiwi (Actinidia arguta)
http://hdl.handle.net/10659/00006694
http://hdl.handle.net/10659/0000669482e0501f-c354-439d-989e-5646979a318d
名前 / ファイル | ライセンス | アクション |
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R-2020-276_miyazaki.pdf (441.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-11-18 | |||||
タイトル | ||||||
タイトル | Purification and Biochemical Characterization of Cysteine Protease from Baby Kiwi (Actinidia arguta) | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Actinidia arguta | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cysteine protease | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Purification | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Biochemichal properties | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Allied species | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chromatography | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Actinidia arguta | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cysteine protease | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Purification | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Biochemichal properties | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Allied species | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Chromatography | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
宮崎, 早花
× 宮崎, 早花× Yoneta-Wada, Mio× Kozuka, Miyuki× Sakaue, Tomohisa× Yamane, Takuya× Suzuki, Junko× Arakawa, Yoshihito× Ohkubo, Iwao |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: It has recently been reported that the fruit, stems and leaves of Actinidia arguta have various potential health effects including an antioxidant effect, anticancer effect, anti-allergic effect and α-glucosidase inhibitory effect. However, little is known about the biochemical properties of cysteine protease in the fruit juice of A. arguta. Methods: Ion exchange chromatography to purify the cysteine protease from the fruit juice of A. arguta, and some synthetic substrates to determinate the enzyme activity were used. Results: Cysteine protease was purified to homogeneity from A. arguta fruit juice by ion exchange chromatography. The molecular weight of the purified enzyme was calculated to be approximately 25,500 by SDS-PAGE in the presence of β-ME. The enzyme rapidly hydrolyzed the substrate Z-Leu-Arg-MCA and moderately hydrolyzed other substrates including Boc-Val-Leu-Lys-MCA, Z-Val-Val-Arg-MCA and Z-Phe-Arg-MCA. Kinetic parameters for these four substrates were determined. The Km, Vmax, Kcat and Kcat/Km values for Z-Leu-Arg-MCA, the most preferentially cleaved by the enzyme, were 100 μM, 63.8 μmoles/mg/min, 27.26 sec-1 and 0.2726 sec-1μM-1, respectively. Furthermore, the activity of the enzyme was strongly inhibited by inhibitors including antipain, leupeptin, E-64, E-64c, kinin-free-LMW kininogen and cystatin C. Those biochemical data indicated that the enzyme was a cysteine protease. The amino acid sequence of the first 21 residues of cysteine protease purified from Actinidia arguta was Val1-Leu-Pro-Asp-Tyr5-Val-Asp-Trp-Arg-Ser10-Ala-Gly-Ala-Val-Val15-Asp-Ile-Lys-Ser-Qln20-Gly. This sequence showed high homology to the sequences of actinidin from Acinidia deliciosa (95.0%) and actinidin from Actinidia eriantha (90%). These three cysteine proteases were thought to be common allied species. Conclusion: The biochemical properties of the enzyme purified from A. arguta fruit juice were determined. These basic data are expected to contribute to the maintenance and improvement of human health as well as to the promotion of protein digestion and absorption through its proteolytic functions. | |||||
書誌情報 |
The Open Biochemistry Journal 巻 13, p. 54-63, 発行日 2019 |
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ISBN | ||||||
識別子タイプ | ISBN | |||||
関連識別子 | 1874-091X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.2174/1874091X01913010054 | |||||
権利 | ||||||
権利情報 | © 2019 Miyazaki-Katamura et al. | |||||
権利(URI) | ||||||
権利情報 | https://creativecommons.org/licenses/by/4.0/legalcode | https://creativecommons.org/licenses/by/4.0/legalcode | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Bentham Open | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |