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Cytochrome P450 Expression and Chemical Metabolic Activity before Full Liver Development in Zebrafish
http://hdl.handle.net/10659/00006981
http://hdl.handle.net/10659/000069819e2d5317-ddb3-4b7c-8f67-42c566baf5d3
名前 / ファイル | ライセンス | アクション |
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R-2021-6_teraoka.pdf (4.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-02-21 | |||||
タイトル | ||||||
タイトル | Cytochrome P450 Expression and Chemical Metabolic Activity before Full Liver Development in Zebrafish | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cytochrome P450 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | drug metabolism | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | developing zebrafish | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cytochrome P450 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | drug metabolism | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | developing zebrafish | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nawaji, Tasuku
× Nawaji, Tasuku× Yamashita, Natsumi× Umeda, Haruka× Zhang, Shuangyi× Mizoguchi, Naohiro× Seki, Masanori× Kitazawa, Takio× TERAOKA, Hiroki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Zebrafish are used widely in biomedical, toxicological, and developmental research, but information on their xenobiotic metabolism is limited. Here, we characterized the expression of 14 xenobiotic cytochrome P450 (CYP) subtypes in whole embryos and larvae of zebrafish (4 to 144 h post-fertilization (hpf)) and the metabolic activities of several representative human CYP substrates. The 14 CYPs showed various changes in expression patterns during development. Many CYP transcripts abruptly increased at about 96 hpf, when the hepatic outgrowth progresses; however, the expression of some cyp1s (1b1, 1c1, 1c2, 1d1) and cyp2r1 peaked at 48 or 72 hpf, before full liver development. Whole-mount in situ hybridization revealed cyp2y3, 2r1, and 3a65 transcripts in larvae at 55 hpf after exposure to rifampicin, phenobarbital, or 2,3,7,8-tetrachlorodibenzo-p-dioxin from 30 hpf onward. Marked conversions of diclofenac to 4′-hydroxydiclofenac and 5-hydroxydiclofenac, and of caffeine to 1,7-dimethylxanthine, were detected as early as 24 or 50 hpf. The rate of metabolism to 4’-hydroxydiclofenac was more marked at 48 and 72 hpf than at 120 hpf, after the liver had become almost fully developed. These findings reveal the expression of various CYPs involved in chemical metabolism in developing zebrafish, even before full liver development. | |||||
書誌情報 |
Pharmaceuticals 巻 13, 号 12, p. 456-1-456-17, 発行日 2020 |
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DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3390/ph13120456 | |||||
権利 | ||||||
権利情報 | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||
権利(URI) | ||||||
権利情報 | http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | MDPI | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |