@article{oai:rakuno.repo.nii.ac.jp:00001243,
 author = {Yaguchi, Shigenori and McCallum, John and Shaw, Martin and Pither-Joyce, Meeghan and Hang, Tran Thi Minh and Tsukazaki, Hikaru and Hoa, Vu Quynh and Masuzaki, Shin-ichi and Wako, Tadayuki and Onodera, Shuichi and Shiomi, Norio and Yamauchi, Naoki and Shigyo, Masayoshi},
 issue = {Special Issue 1},
 journal = {Dynamic Biochemistry, Process Biotechnology and Molecular Biology},
 month = {Dec},
 note = {Article, Onion (Allium cepa L.) and shallot (A. cepa Aggregatum group) exhibit wide variation in bulb fructan content and the Frc locus on chromosome 8 conditions much of this variation. To understand the biochemical basis of Frc we conducted biochemical and genetic analyses of Allium fistulosum (FF) - shallot alien monosomic addition lines (AALs; FF+1 A-FF+8A), onion mapping populations and shallot - A. fistulosum addition lines. Sucrose and fructan levels in leaves of FF+2A were significantly lower than FF throughout the year. FF+8A showed significantly higher winter sucrose accumulation and sucrose phosphate synthase (SPS) activity. Markers for additional candidate genes for sucrose metabolism were obtained by cloning a major SPS expressed in onion leaf and exhaustively mining onion EST resources. SPS and sucrose synthase (SuSy) loci were assigned to chromosome 8 and 6 respectively using AALs and linkage mapping. Further loci were assigned, using AALs, to chromosomes 1 (sucrose phosphate phosphatase), 2 (SuSy and 3 invertases) and 8 (neutral invertase). The shallot - A. fistulosum AAL (AA+8F) also showed the high fructan accumulation. The concordance between chromosome 8 localization of SPS and elevated leaf sucrose levels conditioned by high fructan alleles at the Frc locus in bulb onion or alien monosomic additions of chromosome 8 in A. fistulosum and in A. cepa suggest that the Frc locus may condition variation in SPS activity.},
 pages = {70--77},
 title = {Chromosome Engineering Techniques Modify Contents and Constituents of Fructans in Cultivated Allium  Species},
 volume = {3},
 year = {2009}
}