{"created":"2023-06-19T07:00:04.024249+00:00","id":1243,"links":{},"metadata":{"_buckets":{"deposit":"73894aea-8202-40dc-8bf0-58ab31029dda"},"_deposit":{"created_by":3,"id":"1243","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1243"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00001243","sets":["9:10:18:19"]},"author_link":["2744","2747","2746","2750","2749","2741","2751","2753","2742","2745","2752","2748","2743"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Special Issue 1","bibliographicPageEnd":"77","bibliographicPageStart":"70","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"Dynamic Biochemistry, Process Biotechnology and Molecular Biology"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Onion (Allium cepa L.) and shallot (A. cepa Aggregatum group) exhibit wide variation in bulb fructan content and the Frc locus on chromosome 8 conditions much of this variation. To understand the biochemical basis of Frc we conducted biochemical and genetic analyses of Allium fistulosum (FF) - shallot alien monosomic addition lines (AALs; FF+1 A-FF+8A), onion mapping populations and shallot - A. fistulosum addition lines. Sucrose and fructan levels in leaves of FF+2A were significantly lower than FF throughout the year. FF+8A showed significantly higher winter sucrose accumulation and sucrose phosphate synthase (SPS) activity. Markers for additional candidate genes for sucrose metabolism were obtained by cloning a major SPS expressed in onion leaf and exhaustively mining onion EST resources. SPS and sucrose synthase (SuSy) loci were assigned to chromosome 8 and 6 respectively using AALs and linkage mapping. Further loci were assigned, using AALs, to chromosomes 1 (sucrose phosphate phosphatase), 2 (SuSy and 3 invertases) and 8 (neutral invertase). The shallot - A. fistulosum AAL (AA+8F) also showed the high fructan accumulation. The concordance between chromosome 8 localization of SPS and elevated leaf sucrose levels conditioned by high fructan alleles at the Frc locus in bulb onion or alien monosomic additions of chromosome 8 in A. fistulosum and in A. cepa suggest that the Frc locus may condition variation in SPS activity.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Global Science Books Ltd."}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"http://www.globalsciencebooks.info/Journals/DBPBMB.html | http://www.globalsciencebooks.info/Journals/DBPBMB.html"}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1749-0626","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yaguchi, Shigenori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"McCallum, John"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shaw, Martin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Pither-Joyce, Meeghan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hang, Tran Thi Minh"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsukazaki, Hikaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hoa, Vu Quynh"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuzaki, Shin-ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wako, Tadayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Onodera, Shuichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiomi, Norio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamauchi, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shigyo, Masayoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-14"}],"displaytype":"detail","filename":"S-926_Onodera.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-926_Onodera.pdf","url":"https://rakuno.repo.nii.ac.jp/record/1243/files/S-926_Onodera.pdf"},"version_id":"ed44c0d1-28fd-47a7-8db9-75dfea286d70"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Japanese bunching onion","subitem_subject_scheme":"Other"},{"subitem_subject":"mapping","subitem_subject_scheme":"Other"},{"subitem_subject":"onion","subitem_subject_scheme":"Other"},{"subitem_subject":"shallot","subitem_subject_scheme":"Other"},{"subitem_subject":"sucrose","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Chromosome Engineering Techniques Modify Contents and Constituents of Fructans in Cultivated Allium  Species","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Chromosome Engineering Techniques Modify Contents and Constituents of Fructans in Cultivated Allium  Species"}]},"item_type_id":"2","owner":"3","path":["19"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-03-31"},"publish_date":"2011-03-31","publish_status":"0","recid":"1243","relation_version_is_last":true,"title":["Chromosome Engineering Techniques Modify Contents and Constituents of Fructans in Cultivated Allium  Species"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T09:09:41.713158+00:00"}