{"created":"2023-06-19T07:00:04.065741+00:00","id":1244,"links":{},"metadata":{"_buckets":{"deposit":"6e8a32fd-0d57-4936-8fd3-8fdf01217b3d"},"_deposit":{"created_by":3,"id":"1244","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1244"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00001244","sets":["9:10:18:19"]},"author_link":["2754","2758","2755","2757","2756"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-04","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"16-9","bibliographicPageStart":"16-1","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"Chemistry Central Journal"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: We have previously reported on the variation of total fructooligosaccharides (FOS), total inulooligosaccharides (IOS) and inulin in the roots of burdock stored at different temperatures. During storage at 0°C, an increase of FOS as a result of the hydrolysis of inulin was observed. Moreover, we suggested that an increase of IOS would likely be due to the synthesis of the IOS by fructosyltransfer from 1-kestose to accumulated fructose and elongated fructose oligomers which can act as acceptors for fructan:fructan 1-fructosyltransferase (1-FFT). However, enzymes such as inulinase or fructan 1-exohydorolase (1-FEH) involved in inulin degradation in burdock roots are still not known. Here, we report the isolation and functional analysis of a gene encoding burdock 1-FEH. Results: A cDNA, named aleh1, was obtained by the RACE method following PCR with degenerate primers designed based on amino-acid sequences of FEHs from other plants. The aleh1 encoded a polypeptide of 581 amino acids. The relative molecular mass and isoelectric point (pI) of the deduced polypeptide were calculated to be 65,666 and 4.86. A recombinant protein of aleh1 was produced in Pichia pastoris, and was purified by ion exchange chromatography with DEAE-Sepharose CL-6B, hydrophobic chromatography with Toyopearl HW55S and gel filtration chromatography with Toyopearl HW55S. Purified recombinant protein showed hydrolyzing activity against β-2, 1 type fructans such as 1-kestose, nystose, fructosylnystose and inulin. On the other hand, sucrose, neokestose, 6-kestose and high DP levan were poor substrates. The purified recombinant protein released fructose from sugars extracted from burdock roots. These results indicated that aleh1 encoded 1-FEH.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/1752-153X-5-16","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2011 Ueno et al"},{"subitem_rights":"http://creativecommons.org/licenses/by/2.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ueno, Keiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishiguro, Yojiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Midori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Onodera, Shuichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiomi, Norio"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-14"}],"displaytype":"detail","filename":"S-2012-6_onodera.pdf","filesize":[{"value":"461.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-2012-6_onodera.pdf","url":"https://rakuno.repo.nii.ac.jp/record/1244/files/S-2012-6_onodera.pdf"},"version_id":"91f97c4e-c169-4e6d-b439-60eb9ffee704"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium Iappa L.)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium Iappa L.)"}]},"item_type_id":"2","owner":"3","path":["19"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-06-04"},"publish_date":"2013-06-04","publish_status":"0","recid":"1244","relation_version_is_last":true,"title":["Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium Iappa L.)"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T09:09:40.918377+00:00"}