{"created":"2023-06-19T07:00:04.668955+00:00","id":1258,"links":{},"metadata":{"_buckets":{"deposit":"32ec9f18-31d4-4e7f-8fea-7eff49fd211d"},"_deposit":{"created_by":3,"id":"1258","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1258"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00001258","sets":["9:10:18:19"]},"author_link":["2828","2833","2831","2832","2829","2830"],"item_2_alternative_title_22":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Thermoanaerobacter brockii Kojibiose Phosphorylaseによって合成された新規三および四糖の分離および同定"}]},"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"200","bibliographicPageStart":"195","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"Journal of Applied Glycoscience"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Novel tri- and tetra-saccharides were synthesized by glucosyltransfer from β-D-glucose 1-phosphate (β-D-G1P) to palatinose using Thermoanaerobacter brockii kojibiose phosphorylase. There saccharides were isolated using carbon-Celite column chromatography and preparative high performance liquid chromatography. Gas liquid chromatography analysis of methyl derivatives, MALDI-TOF MS and NMR measurements were used for structural confirmation of the saccharides. The ^1H and ^13C NMR signals of the saccharides were assigned using 2D-NMR including COSY, HSQC, HSQC-TOCSY and HMBC. These oligosaccharides were identified as 2^G-α-D-glucopyranosyl-palatinose; O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl-(1→6)-D-fructofuranose and 2^G(2-α-D-glucopyranosyl)_2-palatinose; O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl-(1→6)-D-fructofuranose. パラチノースは抗う蝕性のようなさまざまな機能をもつことが知られている．しかしながら，パラチノースはゆっくりではあるが小腸で加水分解をうけるため，糖尿病のような疾患をもつ患者への使用は薦められない．さらに，この糖のような低分子のオリゴ糖は比較的高浸透圧になりやすいため生体にとってよくない影響を及ぼすことがある．本研究では，二糖であるパラチノースを用い，Thermoanaerobacter brockii kojibiose phosphorylaseのグルコシル転移作用を利用し，グルコース1リン酸とパラチノースから新規オリゴ糖を合成した．反応は糖1および糖2が効率よく生成した48時間で止めた (Fig. 1)．また，転移生成物である糖1は反応10時間で最大となった (Fig. 2)．活性炭-セライトカラムおよび調製用HPLCを用いて糖1および糖2を単離し，MALDI-TOF-MS分析およびメチル誘導体のガスクロマトグラフィー分析を行い構造の推定を行った (Fig. 3)．さらにCOSY，HSQC，HSQC-TOCSYおよびHMBC (Fig. 4) の各手法を用いた2次元NMR解析により糖1を2^G-α-D-glucopyranosyl-palatinose; O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl- (1→6)-D-fructofuranose，糖2を2^G(2-α-D-glucopyranosyl)_2-palatinose; O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl- (1→2)-O-α-D-glucopyranosyl-(1→6)-D-fructofuranoseと同定した (Table 1)．今後，これらの糖の栄養機能について明らかにする必要がある","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本応用糖質学会"}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1344-7882","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Natsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukushi, Eri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Onodera, Shuichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishimoto, Tomoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawabata, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiomi, Norio"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-14"}],"displaytype":"detail","filename":"S-16_Onodera.pdf","filesize":[{"value":"297.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-16_Onodera.pdf","url":"https://rakuno.repo.nii.ac.jp/record/1258/files/S-16_Onodera.pdf"},"version_id":"b920538b-def6-49ed-9f9a-a7d854bd68ab"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isolation and identification of noble tri- and tetrasaccharides synthesized by Thermoanaerobacter brockii kojibiose phosphorylase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation and identification of noble tri- and tetrasaccharides synthesized by Thermoanaerobacter brockii kojibiose phosphorylase"}]},"item_type_id":"2","owner":"3","path":["19"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-03-11"},"publish_date":"2010-03-11","publish_status":"0","recid":"1258","relation_version_is_last":true,"title":["Isolation and identification of noble tri- and tetrasaccharides synthesized by Thermoanaerobacter brockii kojibiose phosphorylase"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T09:09:24.561612+00:00"}