@article{oai:rakuno.repo.nii.ac.jp:00001267,
 author = {Fukushi, Eri and Okada, Hideki and Yamamori, Akira and Kawazoe, Naoki and Onodera, Shuichi and Kawabata, Jun and Shiomi, Norio},
 issue = {Special Issue 1},
 journal = {Dynamic Biochemistry, Process Biotechnology and Molecular Biology},
 month = {Dec},
 note = {Article, This review focuses on the NMR methods for the oligosaccharides containing fructopyranoside that were previously isolated from the fermented beverage of an extract from 50 kinds of fruits and vegetables. The ^1H and ^<13>C-NMR signals of each saccharide were assigned using 2D-NMR including COSY, HSQC, HSQC-TOCSY, CH_2-selected HSQC-TOCSY, and CT (constant time)-HMBC. The fructose in pyranosyl form showed different ^<13>C chemical shifts from those of furanosyl form. Further confirmation of the pyranosyl form could be obtained from the HMBC correlation peak between C-2 and H-6 of fructose residue (Fru), whereas the C-2 of Fru in furanosyl form chould give the HMBC correlation peak between H-5 of Fru. Problems encountered were signal overlapping of protons and low peak separation. The key correlation peak between C-2 and H-6 of Fru was overlapped by the correlation peak indicating a glycosidic linkage between the C-2 of Fru and the H-6 of the glucose residue (Glc, or Glc’). These were solved using HSQC and CT-HMBC spectra rather than HMQC and conventional HMBC spectra, which have an inherent broad-line-shape in the carbon dimension.},
 pages = {10--15},
 title = {NMR Analysis of Oligosaccharides Containing Fructopyranoside},
 volume = {3},
 year = {2009}
}