@article{oai:rakuno.repo.nii.ac.jp:00001419,
 author = {菅原, 諒太 and 山田, さゆみ and 涂, 志豪 and 菅原, 明子 and 干場, 敏博 and 山内, 正仁 and 山口, 昭弘},
 issue = {9},
 journal = {日本食品科学工学会誌},
 month = {Sep},
 note = {Article, This study focuses on the identification and characterization of wild mushrooms grown on the campus of Rakuno Gakuen University, using DNA sequencing and analysis of functional metabolites. Forty-eight samples of fruiting bodies were collected between June 2012 and November 2014. The samples were hot-air dried and powdered, and subsequently extracted with alkaline hot water to obtain beta-glucans, or 50% ethanol for total polyphenols (TPP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Functional metabolites were assessed by colorimetric assays, using Congo Red reagent for beta-glucans, Folin-Denis reagent for TPP level, and DPPH radical scavenging for antioxidant activity. Total DNA was extracted from each sample and subjected to DNA sequencing for the inter spacer regions of ribosomal RNA gene. Sequencing analysis identified 27 independent species or genus of mushrooms. Beta-glucan contents showed clear differences between species. The TPP levels and DPPH radical scavenging activity showed similar tendencies and were significantly correlated ( p<0.0001). 酪農学園大学キャンパス内の自生キノコ48試料について遺伝子解析による同定を行ったところ46試料が種あるいは属レベルで同定された．Congo Red法によるβ-グルカンの定量結果は，測定値にセルロースなどの直鎖状β-グルカンを含まないことから，酵素法と比べて小さい値となったがβ-グルカンの高次構造を反映した機能性を評価する上ではむしろ有用であると考えられた．また食材の機能性評価に汎用されるTPPおよびDPPHラジカル消去活性を測定したところ，両測定値間には強い正の相関が認められ，キノコの抗酸化性にはポリフェノールが関与していることが示唆された．β-グルカン，TPPおよびDPPHラジカル消去活性のいずれの機能性指標においても科ごとに一定の傾向は見られず，種ごとに特徴的な値を示すことが確認された．},
 pages = {445--453},
 title = {道央圏に自生するキノコの同定と機能性成分の含有量},
 volume = {62},
 year = {2015}
}