{"created":"2023-06-19T07:00:38.530969+00:00","id":2020,"links":{},"metadata":{"_buckets":{"deposit":"bfe19f6a-9dfa-47fc-ae20-13bf47e511a2"},"_deposit":{"created_by":3,"id":"2020","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2020"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00002020","sets":["9:27:28:29"]},"author_link":["4619","4617","4614","4618","4616","4620","4622","4615","4621","4613"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"193","bibliographicPageStart":"191","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"Journal of Veterinary Science"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit for detecting mycoplasma by the simplified PCR was estimated to be about 2.5 × 103 cfu/mL and was similar to that of the standard PCR. We compared the specificity and sensitivity of the simplified PCR to those of a culture method. Out of 1,685 milk samples cultured in mycoplasma broth, the simplified PCR detected Mycoplasma DNA in 152 that were also positive according to the culture assay. The sensitivity and specificity of the simplified PCR were 98.7% and 99.7%, respectively, for detecting mycoplasma in those cultures. The results obtained by the simplified PCR were consistent with ones from standard PCR. This newly developed simplified PCR, which does not require DNA purification, can analyze about 300 cultured samples within 3 h. The results from our study suggest that the simplified PCR can be used for mycoplasma mastitis screening in large-scale dairy farms.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Korean Society of Veterinary Science"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.4142/jvs.2011.12.2.191","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2011 The Korean Society of Veterinary Science"},{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medi"},{"subitem_rights":"http://creativecommons.org/licenses/by-nc/3.0"}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1229-845X","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Higuchi, Hidetoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwano, Hidetomo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawai, Kazuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohta, Takehiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Obayashi, Tetsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirose, Kazuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Nobuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokota, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tamura, Yutaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagahata, Hajime"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"S-2012-179_higuchi.pdf","filesize":[{"value":"610.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-2012-179_higuchi.pdf","url":"https://rakuno.repo.nii.ac.jp/record/2020/files/S-2012-179_higuchi.pdf"},"version_id":"72326095-73f6-45ec-90a7-88c03a8de1b3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"bovine","subitem_subject_scheme":"Other"},{"subitem_subject":"mastitis","subitem_subject_scheme":"Other"},{"subitem_subject":"mycoplasma","subitem_subject_scheme":"Other"},{"subitem_subject":"simplified PCR","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-06-04"},"publish_date":"2013-06-04","publish_status":"0","recid":"2020","relation_version_is_last":true,"title":["A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:58:42.380414+00:00"}