@article{oai:rakuno.repo.nii.ac.jp:00002031,
 author = {NAGAHATA, Hajime and KAWAI, Hiroatsu and 樋口, 豪紀 and KAWAI, Kazuhiro and YAYOU, Kenichi and CHANG, Chai J.},
 issue = {7},
 journal = {The journal of veterinary medical science},
 month = {Jul},
 note = {Article, Changes in inflammatory parameters, leukocyte surface markers, functional responses and cytokine mRNA expression of leukocytes of dairy cows with naturally occurring chronic Staphylococcus aureus (S. aureus) mastitis and healthy cows were determined to elucidate the leukocyte responses to S. aureus infection of the mammary gland. Increased values in inflammatory parameters and matrix metalloproteinase activities in milk revealed the characteristics of cows with chronic mastitis. Expression of L-selectin and CD18 molecules on neutrophils and proportion of CD8 cells in milk from cows with S. aureus mastitis were significantly (P<0.05) increased compared with those found in healthy cows. The FcR-stimulated CL response of blood neutrophils was significantly (P<0.05) decreased in cows with S. aureus mastitis. Significantly (P<0.05) decreased mitogenic responses of lymphocytes were found in cows with S. aureus mastitis; however, the values were not restored to those of healthy cows when stimulated with both mitogens and the cytokine IL-1β. The mRNA expression of TNF-α, IL-1β and IL-8 on milk leukocytes from cows with S. aureus was found to be increased compared with that of healthy cows. The changes of immune responses found in cows with S. aureus mastitis appear to be influenced by the severity and duration of inflammation in infected quarters. The down-regulation of the leukocyte functions found in cows with S. aureus mastitis appears to be associated with the progress of the chronic stage of S. aureus mastitis.},
 pages = {885--894},
 title = {Altered Leukocyte Responsiveness in Dairy Cows with Naturally Occurring Chronic Staphylococcus aureus Mastitis},
 volume = {73},
 year = {2011}
}