{"created":"2023-06-19T07:00:39.754818+00:00","id":2049,"links":{},"metadata":{"_buckets":{"deposit":"211b10a3-a298-466a-8503-4ac2493063b7"},"_deposit":{"created_by":3,"id":"2049","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2049"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00002049","sets":["9:27:28:29"]},"author_link":["4770","4772","4767","4775","4768","4771","4774","4769","4773"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"e0117795-12","bibliographicPageStart":"e0117795-1","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"PLOS ONE"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Corticosterone is synthesized in the adrenal glands and is circulated throughout the body to perform regulatory functions in various tissues. The testis is known to synthesize and secrete testosterone and other androgens. We developed an accurate method to measure steroid content using liquid chromatography-mass spectrometry analysis. In the present study, significant levels of the precursor compounds of testosterone and corticosterone synthesis could be detected in rat testis using this method. After adrenalectomy, corticosterone remained in the blood and testicular tissue at approximately 1% of the amount present in the control testis. When the excised testicular tissue was washed and incubated with NADH, NADPH and progesterone, not only testosterone and its precursors but also 11-deoxycorticosterone and corticosterone were produced; the levels of 11-deoxycorticosterone and corticosterone increased with incubation time. The production rate of 11-deoxycorticosterone from progesterone was estimated to be approximately 1/20 that of 17-hydroxyprogesterone, and the corticosterone level was approximately 1/10 that of testosterone. These ratios coincided with those in the testicular tissue of the adrenalectomized rats, indicating that corticosterone was synthesized in the testis and not in the blood. A primary finding of this study was that corticosterone and testosterone were synthesized in a 1/10-20 ratio in the testis. It is concluded that corticosterone, which has various functions, such as the regulation of glycolysis and mediating spermatogenesis, is produced locally in the testis and that this the local production is convenient and functional to respond to local needs.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0117795","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright: © 2015 Maeda et al."},{"subitem_rights":"This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by/4.0/ | https://creativecommons.org/licenses/by/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Maeda, Naoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tahata, Sachi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yagi, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Emi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masu, Kanae"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Michiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Haeno, Satoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Onaga, Takenori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokota, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"S-2016-178_onaga.pdf","filesize":[{"value":"873.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-2016-178_onaga.pdf","url":"https://rakuno.repo.nii.ac.jp/record/2049/files/S-2016-178_onaga.pdf"},"version_id":"ff171848-5e4b-489a-b3f0-8041c064320c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Assessment of Testicular Corticosterone Biosynthesis in Adult Male Rats","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Assessment of Testicular Corticosterone Biosynthesis in Adult Male Rats"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-06-09"},"publish_date":"2017-06-09","publish_status":"0","recid":"2049","relation_version_is_last":true,"title":["Assessment of Testicular Corticosterone Biosynthesis in Adult Male Rats"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:58:11.010298+00:00"}