@article{oai:rakuno.repo.nii.ac.jp:00002140,
 author = {ONUMA, Manabu and ZHAO, Chen and 浅川, 満彦 and ASAKAWA, Mitsuhiko and NAGAMINE, Takashi and KUWANA, Takashi},
 issue = {1},
 journal = {日本野生動物医学会誌},
 month = {Mar},
 note = {Article, A duplex real-time polymerase chain reaction (PCR) assay was developed to decrease expended time for diagnosis as well as to improve on the sensitivity and specificity of current molecular diagnostic methods for the detection and differentiation of Heterakis isolonche and Glaphyrostomum sp. in Okinawa rail (Gallirallus okinawae). Novel PCR primers and probes were designed based on cytochrome c oxidase subunit I gene. Our results indicated that the assay could simultaneously detect both species without cross-amplification of Okinawa rail DNA. The assay detected DNA from a single parasite egg for both species and successfully amplified DNA extracted from feces. Thus, this new assay is an useful technique for monitoring the levels of parasite infection in Okinawa rail captive breeding program.  ヤンバルクイナ(Gallirallus okinawae)の消化管内寄生虫2種についてDuplex real-time PCR assayを開発した。本法はヤンバルクイナ由来のDNAの影響を受けずに2種を同時に検出できることを確認した。また,寄生虫卵1個分のDNA量も検出でき,糞便にも応用可能であった、,本法はヤンバルクイナ飼育個体群を対象として寄生虫検査を糞便サンプルで実施する場合に特に有用な手法である。},
 pages = {27--31},
 title = {Duplex Real-time PCR Assay for the Detection of Two Intestinal Parasites, Heterakis isolonche and Glaphyrostomum sp., in Okinawa Rail (Gallirallus okinawae)},
 volume = {17},
 year = {2012}
}