{"created":"2023-06-19T07:00:43.776553+00:00","id":2145,"links":{},"metadata":{"_buckets":{"deposit":"75b6d33a-031e-411e-b411-c808c539af3a"},"_deposit":{"created_by":3,"id":"2145","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2145"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00002145","sets":["9:27:28:29"]},"author_link":["5363","5367","5365","5364","5366"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"53-6","bibliographicPageStart":"53-1","bibliographicVolumeNumber":"4","bibliographic_titles":[{"bibliographic_title":"Frontiers in Microbiology"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Understanding the impact of antimicrobial use on the emergence of resistant bacteria is imperative to prevent its emergence. For instance, activation of the AcrAB efflux pumps is responsible for the emergence of antimicrobial-resistant Salmonella strains. Here, we examined the expression levels of acrB and its multiple regulator genes (RamA, SoxS, MarA, and Rob) in 17 field isolates of S. Choleraesuis by using quantitative PCR methods. The expression of acrB increased in eight of the field isolates (P < 0.05). The expression of acrB was associated with that of ramA in one isolate, soxS in one isolate, and both these genes in six isolates. Thereafter, to examine the effect of selected antimicrobials (enrofloxacin, ampicillin, oxytetracycline, kanamycin, and spectinomycin) on the expression of acrB and its regulator genes, mutants derived from five isolates of S. Choleraesuis were selected by culture on antimicrobial-containing plates. The expression of acrB and ramA was higher in the mutants selected using enrofloxacin (3.3-6.3- and 24.5-37.7-fold, respectively), ampicillin (1.8-7.7- and 16.1-55.9-fold, respectively), oxytetracycline (1.7-3.3- and 3.2-31.1-fold, respectively), and kanamycin (1.6-2.2- and 5.6-26.4-fold, respectively), which are AcrAB substrates, than in each of the parental strains (P < 0.05). In contrast, in AcrAB substrate-selected mutants, the expression of soxS, marA, and rob remained similar to that in parental strains. Of the four antimicrobials, the level of ramA expression was significantly higher in the enrofloxacin- and ampicillin-selected mutants than in the oxytetracycline- and kanamycin-selected mutants (P < 0.05), whereas the expression levels of acrB and multiple regulator genes in spectinomycin-selected mutants were similar to those in each parental strain. These data suggest that exposure to antimicrobials that are AcrAB substrates enhance the activation of the AcrAB efflux pump via RamA, but not via SoxS, MarA, or Rob in S. Choleraesuis.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers Media S.A."}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3389/fmicb.2013.00053","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 Usui, Nagai, Hiki, Tamura and Asai. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source a"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Usui, Masaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagai, Hidetaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hiki, Mototaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tamura, Yutaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asai, Tetsuo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"S-2014-153_tamura.pdf","filesize":[{"value":"863.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-2014-153_tamura.pdf","url":"https://rakuno.repo.nii.ac.jp/record/2145/files/S-2014-153_tamura.pdf"},"version_id":"60747953-020c-44dd-bbdd-e2b2da0f271a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AcrAB","subitem_subject_scheme":"Other"},{"subitem_subject":"efflux pump","subitem_subject_scheme":"Other"},{"subitem_subject":"antimicrobial resistance","subitem_subject_scheme":"Other"},{"subitem_subject":"RamA","subitem_subject_scheme":"Other"},{"subitem_subject":"Salmonella Choleraesuis","subitem_subject_scheme":"Other"},{"subitem_subject":"SoxS","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effect of Antimicrobial Exposure on AcrAB Expression in Salmonella enterica Subspecies enterica Serovar Choleraesuis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of Antimicrobial Exposure on AcrAB Expression in Salmonella enterica Subspecies enterica Serovar Choleraesuis"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-12"},"publish_date":"2015-05-12","publish_status":"0","recid":"2145","relation_version_is_last":true,"title":["Effect of Antimicrobial Exposure on AcrAB Expression in Salmonella enterica Subspecies enterica Serovar Choleraesuis"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:56:44.077030+00:00"}