{"created":"2023-06-19T07:00:46.176434+00:00","id":2202,"links":{},"metadata":{"_buckets":{"deposit":"435535ef-2f88-495d-a53b-ddb355bedf51"},"_deposit":{"created_by":3,"id":"2202","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2202"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00002202","sets":["9:27:28:29"]},"author_link":["5723","5728","5726","5724","5729","3287","5727","5725","5722","5730"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"115","bibliographicPageStart":"111","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"The Journal of Veterinary Medical Science"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cellular drug resistance to antineoplastic drugs is often due to the presence of a drug efflux pump that reduces intracellular drug accumulation and chemosensitivity. P-glycoprotein (P-gp), which is encoded by the MDR1 gene, is considered to function as an ATP-driven membrane drug efflux pump and appears to play an important role in tumor cell resistance. In the present report, we assessed the expression of MDR1 by RT-PCR in three canine mast cell tumor cell lines, TiMC, CoMS and LuMC, originating from a cutaneous tumor, an oral-mucosal tumor and a gastrointestinal tumor, respectively. P-gp expression was also examined by Western blot analysis, while the functional activity of P-gp was assessed by flowcytometric analysis of intracellular rhodamine-123 (Rhd-123) uptake. The results revealed that MDR1 gene and P-gp were both expressed in CoMS and LuMC cells, whereas neither was present in TiMC cells. In CoMS and LuMC cells, intracellular uptake of Rhd-123 increased in the presence of verapamil, a functional modulator of P-gp. In contrast, TiMC cells did not show any changes in the intracellular accumulation of Rhd-123 after the verapamil addition. These findings suggest that the expressions of MDR1 gene and P-gp probably contribute to cellular drug resistance in canine mast cell tumors.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本獣医学会"}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-7250","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAKAICHI, Munekazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAKESHITA, Yoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OKUDA, Masaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NAKAMOTO, Yuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ITAMOTO, Kazuhito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"UNE, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SASAKI, Nobuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KADOSAWA, Tsuyoshi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"TAKAHASHI, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAURA, Yasuho"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"S-172_Kadosawa.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-172_Kadosawa.pdf","url":"https://rakuno.repo.nii.ac.jp/record/2202/files/S-172_Kadosawa.pdf"},"version_id":"2e20b385-3688-47f8-822d-8b8cf579a0d1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-03-11"},"publish_date":"2010-03-11","publish_status":"0","recid":"2202","relation_version_is_last":true,"title":["Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:55:43.267660+00:00"}