@article{oai:rakuno.repo.nii.ac.jp:00002224,
 author = {ENDO, Kentaro and ITOH, Norihiko and MAEHARA, Seiya and SHINOZAKI, Aya and IMAGAWA, Tomohiro and UEHARA, Masato and MIZUNO, Naoharu and SASAKI, Shoji and HIRAGA, Takeo and TERAOKA, Hiroki},
 issue = {2},
 journal = {The journal of veterinary medical science},
 month = {Feb},
 note = {Article, Manganese deficiency results in neurological and skeletal defects, together with ultrastructural disarrangement of the retina in rats. Wild birds show a range of Mn concentrations in their tissues, including the liver, raising the possibility of Mn-related disorders in the wild. Electroretinography (ERG) provides a useful noninvasive approach to evaluate visual function. This method is especially useful in birds, as objective analysis of them is very difficult, while they have well-developed vision. In this study, we carried out a convenient and reliable ERG recording using a contact lens electrode with a built-in light source (LED electrode) of Japanese quail (Coturnix coturnix japonica) fed a Mn-deficient diet. After 10 min light adaptation, single-flash and flicker cone responses were reproducibly recorded to cause an intensity-dependent increase in amplitude of both a-wave and b-wave in single-flash ERG. Mn-deficient feeding markedly decreased the Mn concentration in the liver by almost half in 3 to 6 weeks, followed by body weight loss in 13 to 15 weeks. Implicit time of a-wave and b-wave cone response by single-flash stimulation was significantly delayed in quail with a Mn depletion from 3 to 6 weeks. Every cone response of the Mn-deprived quail had a tendency to decrease amplitude. The ultrastructure of cone photoreceptor cells was disorganized by Mn deficiency, including changes in outer segment discs of photoreceptor cells. These results suggest the essential role of Mn in the integrity of the retinal function of birds.},
 pages = {139--144},
 title = {Functional Disorder of the Retina in Manganese-Deficient Japanese Quail Revealed by Electroretinography using a Contact Lens Electrode with Built-In Light Source},
 volume = {70},
 year = {2008}
}