{"created":"2023-06-19T07:00:49.536719+00:00","id":2282,"links":{},"metadata":{"_buckets":{"deposit":"811300c8-be4a-4616-a2e0-854b909a6e5f"},"_deposit":{"created_by":3,"id":"2282","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2282"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00002282","sets":["9:27:28:29"]},"author_link":["4684","4683","6242","6244","6243","6247","6245","6246"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"945","bibliographicPageStart":"937","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":"The Journal of Veterinary Medical Science"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Understanding the prevalence of antimicrobial-resistance and the relationship between emergence of resistant bacteria and clinical treatment can facilitate design of effective treatment strategies. We here examined antimicrobial susceptibilities of Escherichia coli isolated from dogs admitted to a university hospital (University hospital) and companion animal clinics (Community clinics) in the same city and investigated underlying multidrug-resistance mechanisms. The prevalence of E. coli with intermediate and resistant interpretations to ampicillin (AMP), enrofloxacin (ENR) and chloramphenicol (CHL) was higher in the University hospital than in the Community clinics cases. Use of antimicrobials, including fluoroquinolone, was also significantly higher in the University hospital than in the Community clinics cases. Upon isolation using ENR-supplemented agar plates, all ENR-resistant isolates had 3-4 nucleotide mutations that accompanied by amino acid substitutions in the quinolone-resistance-determining regions of gyrA, parC and parE, and 94.7% of all isolates derived from the University hospital showed AMP and/or CHL resistance and possessed bla_<TEM> and/or catA1. The average mRNA expression levels of acrA, acrB and tolC and the prevalence of organic solvent tolerance, in isolates derived from ENR-supplemented agar plates were significantly higher in the University hospital than in the Community clinics isolates. Thus, E. coli derived from the University hospital cases more often showed concomitant decreased susceptibilities to aminopenicillins, fluoroquinolones and CHL than did those derived from the Community clinics; this was related to an active AcrAB-TolC efflux pump, in addition to acquisition of specific resistance genes and genetic mutations.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本獣医学会"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1292/jvms.13-0144","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-7250","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SATO, Toyotaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YOKOTA, Shin-ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ICHIHASHI, Risa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIYAUCHI, Tomoka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OKUBO, Torahiko"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"USUI, Masaru","creatorNameLang":"en"},{"creatorName":"臼井, 優","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"FUJII, Nobuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAMURA, Yutaka"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"S-2015-248_tamura.pdf","filesize":[{"value":"543.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-2015-248_tamura.pdf","url":"https://rakuno.repo.nii.ac.jp/record/2282/files/S-2015-248_tamura.pdf"},"version_id":"8586da34-68c4-4c62-b707-6f53585adc2c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AcrAB","subitem_subject_scheme":"Other"},{"subitem_subject":"antimicrobial resistance","subitem_subject_scheme":"Other"},{"subitem_subject":"canine","subitem_subject_scheme":"Other"},{"subitem_subject":"efflux pump","subitem_subject_scheme":"Other"},{"subitem_subject":"Escherichia coli","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isolation of Escherichia coli Strains with AcrAB-TolC Efflux Pump-Associated Intermediate Interpretation or Resistance to Fluoroquinolone, Chloramphenicol and Aminopenicillin from Dogs Admitted to a University Veterinary Hospital","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation of Escherichia coli Strains with AcrAB-TolC Efflux Pump-Associated Intermediate Interpretation or Resistance to Fluoroquinolone, Chloramphenicol and Aminopenicillin from Dogs Admitted to a University Veterinary Hospital"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-05-23"},"publish_date":"2016-05-23","publish_status":"0","recid":"2282","relation_version_is_last":true,"title":["Isolation of Escherichia coli Strains with AcrAB-TolC Efflux Pump-Associated Intermediate Interpretation or Resistance to Fluoroquinolone, Chloramphenicol and Aminopenicillin from Dogs Admitted to a University Veterinary Hospital"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T01:58:13.418680+00:00"}