{"created":"2023-06-19T07:00:53.267643+00:00","id":2370,"links":{},"metadata":{"_buckets":{"deposit":"c68331e3-d39e-4100-8a4a-a6bcbae4edb6"},"_deposit":{"created_by":3,"id":"2370","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2370"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00002370","sets":["9:27:28:29"]},"author_link":["6861","6863","6864","6862","6865","6860"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"1203","bibliographicPageStart":"1196","bibliographicVolumeNumber":"118","bibliographic_titles":[{"bibliographic_title":"Environmental Health Perspectives"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background : Bisphenol A (BPA), a well-known endocrine disruptor, is highly glucuronidated in the liver, and the resultant BPA-glucuronide (BPA-GA) is excreted primarily into bile. However, in rodents, prenatal exposure to low doses of BPA can adversely affect the fetus, despite the efficient drug-metabolizing systems of the dams. The transport mechanisms of BPA from mother to fetus are unknown. Objectives : To test our hypothesis that BPA-GA—an inactive metabolite—is passed through the placenta to the fetus, where it affects the fetus after reactivation, we investigated the placental transfer of BPA-GA and reactivation to BPA in the fetus. Methods : After performing uterine perfusion with BPA-GA in pregnant rats, we examined the expression and localization of the placental transporters for drug metabolites in the perfusate by reverse-transcriptase polymerase chain reaction and immunohistochemistry. We also investigated the deconjugation of BPA-GA in the fetus and examined uridine 5′-diphospho-glucuronosyltransferase (UGT) activity toward BPA and the expression of UGT isoforms in fetal liver. Results : We detected BPA-GA and deconjugated BPA in the fetus and amniotic fluid after perfusion. In the trophoblast cells, organic anion-transporting polypeptide 4a1 (Oatp4a1) was localized on the apical membrane, and multidrug resistance-associated protein 1 (Mrp1) was localized to the basolateral membrane. We observed deconjugation of BPA-GA in the fetus; furthermore, we found the expression of UGT2B1, which metabolizes BPA, to be quite low in the fetus. Conclusions : These results demonstrate that BPA-GA is transferred into the fetus and deconjugated in the fetus because of its vulnerable drug-metabolizing system.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Environmental Health Perspectives"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1289/ehp.0901575","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Reproduced with permission from Environmental Health Perspectives"}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0091-6765","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nishikawa, Miyu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwano, Hidetomo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagisawa, Risa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koike, Nanako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokota, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"S-2011-113_Iwano.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-2011-113_Iwano.pdf","url":"https://rakuno.repo.nii.ac.jp/record/2370/files/S-2011-113_Iwano.pdf"},"version_id":"588ef727-e275-452e-b351-a7de37abd266"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"bisphenol A","subitem_subject_scheme":"Other"},{"subitem_subject":"endocrine disruptors","subitem_subject_scheme":"Other"},{"subitem_subject":"estrogenic compounds","subitem_subject_scheme":"Other"},{"subitem_subject":"metabolism","subitem_subject_scheme":"Other"},{"subitem_subject":"transplacental","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Placental transfer of conjugated bisphenol A and subsequent reactivation in the rat fetus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Placental transfer of conjugated bisphenol A and subsequent reactivation in the rat fetus"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-10-01"},"publish_date":"2012-10-01","publish_status":"0","recid":"2370","relation_version_is_last":true,"title":["Placental transfer of conjugated bisphenol A and subsequent reactivation in the rat fetus"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:52:58.998852+00:00"}