{"created":"2023-06-19T07:00:54.461729+00:00","id":2398,"links":{},"metadata":{"_buckets":{"deposit":"969045b7-b46d-4fcd-84e0-93905cc3406e"},"_deposit":{"created_by":3,"id":"2398","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2398"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00002398","sets":["9:27:28:29"]},"author_link":["7006","7005","7003","7004"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-09","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"59-5","bibliographicPageStart":"59-1","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"Acta Veterinaria Scandinavia"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[Background] Infection with Salmonella enterica is a major public health concern in developed countries, and multidrug-resistant strains have become increasingly prevalent. S. enterica serovar Typhimurium DT104 (DT104) strains are prevalent in livestock in Japan and include numerous strains of multidrug-resistant S. enterica. Epidemiological analysis of these strains is critical for both agriculture and public health; however, diagnostic tests for these strains have yielded inconsistent results [Results] We developed a rapid, simple, and inexpensive polymerase chain reaction test to detect multi-drug resistant DT104 strains. We designed primers specific to the prophage ST104 sequence encoded by DT104 strains and assessed the specificity of these primers by assaying a panel of 50 S. enterica isolates. Amplification products of the expected size were generated from the genomes of each of the DT104 strains; however, the ST104 primers failed to amplify products from non-DT104 strains of S. enterica serovar Typhimurium or other S. enterica serovars. Furthermore, a probe generated using the ST104 primers detected a restriction fragment encoding the ST104 region of DT104 by Southern hybridization. [Conclusions] The ST104 primers exhibit specificity to DT104 strains and are suitable for epidemiological applications.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s13028-015-0143-x","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated."}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/"}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1751-0147","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yukawa, Shoichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tamura, Yutaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Kiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uchida, Ikuo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"S-2016-135_tamura.pdf","filesize":[{"value":"984.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-2016-135_tamura.pdf","url":"https://rakuno.repo.nii.ac.jp/record/2398/files/S-2016-135_tamura.pdf"},"version_id":"bc9e994f-a21d-4d69-85b7-d02493c176a9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Detection","subitem_subject_scheme":"Other"},{"subitem_subject":"Epidemiologic investigation","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"Salmonella Typhimurium DT104","subitem_subject_scheme":"Other"},{"subitem_subject":"ST104","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Rapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Rapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-06-09"},"publish_date":"2017-06-09","publish_status":"0","recid":"2398","relation_version_is_last":true,"title":["Rapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:52:26.072163+00:00"}