{"created":"2023-06-19T07:00:57.667172+00:00","id":2474,"links":{},"metadata":{"_buckets":{"deposit":"740111ed-3e8d-4ff0-8fbb-ed6d99c95458"},"_deposit":{"created_by":3,"id":"2474","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2474"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00002474","sets":["9:27:28:29"]},"author_link":["7501","7498","7496","7499","7495","7500","7494","7497"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"468","bibliographicPageStart":"459","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":"Journal of Dairy Research"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Interactions between inflammatory cytokines and plasminogen (Pg) activation system on immune cells are yet to be established. In previous studies we reported a somatic cell-associated elevation of proteolytic activity in mammary secretion of drying-off goats and cows. The purposes of the present study were to examine the role of TNF-α in polymorphonuclear neutrophil (PMN)-associated Pg activation, and the significance of this activation pathway for overall plasmin (Pm) activity in mammary secretion of drying-off cows. Results of experiments in vitro showed that the spontaneous Pg activation observed on fresh preparations of bovine blood PMN was completely blocked by anti bovine TNF-α antibody, and was further up-regulated by exogenous bovine TNF-α. Monitoring the parameters of mammary secretion of drying-off cows revealed that both somatic cell counts and differential PMN ratio was significantly elevated at weeks 1, 2 and 3 of milk stasis. Nevertheless, specific activity of soluble Pm in mammary secretion increased and the level of 17-kDa TNF-α decreased immediately following milk stasis. Iimmunoblotting revealed that although both 26-kDa pro-TNF-α and 17-kDa TNF-α were consistently present in somatic cells of mammary secretion collected at weeks 0, 1, 2 and 3 of milk stasis, only 26-kDa pro-TNF-α was present in somatic cells of milk during lactation. In-vitro assay indicated that cell-free mammary secretion of drying-off cows exerted no Pg activation bioactivity towards bovine blood PMN. Altogether, the current study suggests the existence of an active TNF-α-Pg-Pm autocrine/paracrine loop on the massively infiltrated PMN inside udders of drying-off cows, which involves extensive binding and internalization of 17-kDa TNF-α on PMN and consequently activation of Pg, resulting in high Pm activity and low 17-kDa TNF-α level in mammary secretion. These coordinated mechanisms may play a role in the defence of drying-off mammary gland.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Cambridge University Press"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1017/S0022029909990069","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Proprietors of Journal of Dairy Research 2009"}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"http://journals.cambridge.org/action/displayJournal?jid=DAR | http://journals.cambridge.org/action/displayJournal?jid=DAR"}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-0299","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chou, Wen K"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yu, Ting C"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chen, Shuen E"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Peh, Ho C"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Liu, Wen B"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chen, Ming T"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagahata, Hajime"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chang, Chai J"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"S-735_Nagahata.pdf","filesize":[{"value":"310.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S-735_Nagahata.pdf","url":"https://rakuno.repo.nii.ac.jp/record/2474/files/S-735_Nagahata.pdf"},"version_id":"1ab52dbf-c400-4a31-8915-87c3e8981a8d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"TNFα-mediated plasminogen activation on neutrophils is involved in the high plasmin activity in mammary secretion of drying-off cows","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"TNFα-mediated plasminogen activation on neutrophils is involved in the high plasmin activity in mammary secretion of drying-off cows"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-03-31"},"publish_date":"2011-03-31","publish_status":"0","recid":"2474","relation_version_is_last":true,"title":["TNFα-mediated plasminogen activation on neutrophils is involved in the high plasmin activity in mammary secretion of drying-off cows"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:51:04.267421+00:00"}