@article{oai:rakuno.repo.nii.ac.jp:00002559,
 author = {中村, 正明 and 小山, 毅 and 松井, 義貴 and 平井, 綱雄 and 南橋, 昭 and NAKADA, Ken and 中田, 健 and 横田, 博 and 宮本, 亨},
 issue = {9},
 journal = {日本獣医師会雑誌},
 month = {Sep},
 note = {Article, ハプトグロビン（Hp）測定法の一種であるヘモグロビン結合アッセイ（HBA）を改良し短時間で測定可能なHBA を開発した．本法は一元免疫拡散法と高い相関を示し，再現性も良好であった．健康牛の血中Hp 濃度は分娩後3 日目で最も高く，以後減少した．分娩後6 週から16 週における健康牛の血液（n ＝ 754）の中央値98μg/ml（平均値110μg/ml）であり，95 パーセンタイル値は170μg/ml であった．健康な育成牛（n ＝ 41）では中央値88μg/ml（平均値91μg/ml）であった．また分娩直後に発症した急性乳房炎，胎盤停滞牛は健康牛よりもHp が高く，改良HBA によるHp 測定は分娩後の乳牛における疾病診断の一助に有用と考えられた． Hemoglobin Binding Assay (HBA), one of the methods of bovine serum haptoglobin (Hp) analysis, was modified to shorten the reaction time. The values of bovine serum Hp determined by modified HBA highly correlated with those determined by single radial immunodiffusion assay, and the assay reproducibility (intra- and inter-assay) was acceptable. In healthy postpartum cows, the concentration of serum Hp was elevated until 3 days after parturition and subsequently decreased. The values of the mean, median, and 95th percentile in healthy cows (6h16 weeks after parturition, n = 754) were 110, 98, and 170 μg/ml, respectively. The median value in healthy heifers (n = 41) was 88 μg/ml (mean 91 μg/ml). The levels of serum Hp in cows suffering from post parturient mastitis and a retained placenta were significantly higher than those in healthy cows (1 and 3 days after parturition). Serum Hp was significantly high in cows with acute mastitis or clinical mastitis, compared to those in healthy cows. These results suggest that the modified HBA could be a useful method for diagnosis in postpartum cows.},
 pages = {682--688},
 title = {ヘモグロビン結合アッセイの改良及び乳牛の分娩後における牛ハプトグロビン濃度推移},
 volume = {65},
 year = {2012}
}