@article{oai:rakuno.repo.nii.ac.jp:00002572,
 author = {大塚, 浩子 and 大沼, 学 and 福本, 真一郎 and 向井, 猛 and 白水, 彩 and 千葉, 司 and 浅川, 満彦 and ASAKAWA, Mitsuhiko},
 issue = {1},
 journal = {日本野生動物医学会誌, Japanese journal of zoo and wildlife medicine},
 month = {Mar},
 note = {Article, On November 13, 2000, an Asian tapir (Tapirus indicus) was transferred from the Melaka Zoo in Malaysia to the Sapporo Maruyama Zoo in Japan. During quarantine at the Sapporo Maruyama Zoo, nematode and cestode eggs were observed and disinfestation was conducted using Praziquantel. Segments were found in feces, but it was impossible to identify the cestode species morphologically because all were gravid segments filled with eggs. DNA extraction was conducted from the parasite eggs in the feces and from the segments. Sequences of cytochrome c oxidase subunit 1 (COX1) in mitochondrial DNA and internal transcribed spacer (ITS) were ascertained. The results of a homology search showed that the sequences of ITS obtained from both the parasite eggs and the segments were similar to the sequence of a fungus. Two kinds of COX 1 sequence were obtained from the parasite eggs; one was similar to Ancylostoma, while the other, a sequence that was the same as that obtained from the segments, was related to Paranoplocephala. The results of the present study indicated that species identification might be possible based on sequences obtained from parasite eggs in feces. Furthermore, the method might be useful for diagnosis of parasite infections in wild species with only scarce information about parasitic helminth fauna. マラッカ動物園(マレーシア)より札幌市円山動物園に搬入された一頭のマレーバク(Tapirus indicus)の糞便検査を行ったところ,線虫卵と多数の条虫卵を検出した。プラジクアンテル投与後,糞中より条虫片節が採取されたが老熟片節であった。そこで,糞便中の虫卵および駆虫後排泄された片節からDNAを抽出し,ミトコンドリアのcytochrome c oxidase subunit 1(COX1)遺伝子および核ゲノム中に存在するInternal transcrbed spacer領域(ITS)を対象にPCRを行った。このとき,条虫類と線虫類に共通して使用できるプライマーを用いた。その後PCR産物の塩基配列を決定し,BLASTによるホモロジー検索を行った。その結果,ITS領域用プライマーによって得られた,虫卵および片節由来のPCR産物はすべて真菌類ITSの配列と類似していた。一方,COX1用プライマーによって得られた虫卵由来のPCR産物からは2種類の配列が得られ,一つは裸頭条虫科(Anoplocephalidae)のParanoploaephala属に,もう一つは鈎虫科(Ancylostomidae)のAncylostoma属に近縁の配列であった。このことから本研究において応用された方法は,寄生嬬虫相の情報が少ない野生動物の寄生嬬虫症診断法として有用である可能性が示唆された。},
 pages = {31--37},
 title = {マレーバク(Tapirus indicus)糞便中に認められた虫卵のCOX1およびITS領域塩基配列を指標とした寄生蠕虫類同定の試み},
 volume = {9},
 year = {2004}
}