@article{oai:rakuno.repo.nii.ac.jp:00003188,
 author = {塚田, 努 and 小島, アンナゆみこ and 森好, 政晴 and 古家後, 雅典 and 佐藤, 邦忠 and 澤向, 豊},
 issue = {1},
 journal = {日本家畜臨床学会誌},
 month = {Apr},
 note = {Article, 馬黄体のPGF_<2α>あるいはhCG製剤投与後の反応を解明するため､形成期および開花期黄体組織にMicrodialysis System (MDS) 法を応用し､hCG､PGF_<2α>および血管作動性ペプチド感作後のプロジェステロン(P_4)､PGF_<2α>分泌能を検討した｡その結果､形成期および開花期黄体組織にhCG10 IU､あるいは100 IUを環流してもP_4分泌は刺激されなかった｡しかし､形成期黄体組織のPGF_<2α>産生は対照群と比較し､有意な上昇を認めた(p＜0.05)｡また､開花期黄体組織では100 IU感作群が10IU感作群および対照群と比べ､PGF_<2α>産生が有意に高かった(p＜0.05)｡PGF_<2α>を感作すると､P_4分泌が形成期､開花期黄体組織のいずれにおいても対照群と比べ､有意な上昇を示した(p＜0.05)｡血管作動性ペプチドであるAngiotensin-Ⅱ, Endothelin-ⅠおよびAtrial Natriuretic Peptideの還流では形成期ならびに開花期黄体組織のP_4分泌に有意差が認められなかったが､PGF_<2α>分泌はいずれの組織とも対照群と比べ､有意な上昇を示した(p＜0.05)｡以上の結果から､馬の形成期､あるいは開花期黄体組織にhCG､あるいは血管作動性ペプチドを還流してもP_4分泌を刺激することはなかったが､PGF_<2α>分泌には強く影響することが示唆された｡ The effect of perfusion of hCG, PGF_<2α>, vasoactive peptides, and Ringer's solution (control) on the ovaries of mares (Percheron, Breton and crossbred) during early and functional luteal phases of the ovarian cycle was studied using an in vitro microdialysis system. There was no increase in P_4 secretion as a result of the perfusion of 10 IU or 100 IU of hCG during the early or functional luteal phase. But both the levels of hCG significantly increased PGF_<2α> release, compared to the control, during early luteal phase (p<0.05). During functional luteal phase, 100 IU hCG caused significantly higher secretion of PGF_<2α> than 10 IU hCG or the control (p<0.05). P_4 release was significantly higher than in the control (p<0.05) with PGF_<2α> perfusion in both the early and the functional luteal phases. Vasoactive peptides (angiotensin-Ⅱ, endothelin-Ⅰ and atrial natriuretic peptide) did not cause a significant difference in P_4 release during the early or the functional luteal phase. However, PGF_<2α> release was significantly increased compared to the control (p<0.05) in both phases. These results suggest that hCG and vasoactive peptides had a profound effect on PGF_<2α> release but did not stimulate P_4 secretion during the early or the functional luteal phase in equines.},
 pages = {6--11},
 title = {馬黄体組織のhCG, PGF_<2α>および血管作動性ペプチド還流操作によるProgesteroneとPGF_<2α>分泌動態},
 volume = {31},
 year = {2008}
}