@article{oai:rakuno.repo.nii.ac.jp:00003643,
 author = {Miura, Ryuichi and Kooriyama, Takanori and Yoneda, Misako and Takenaka, Akiko and Doki, Miho and Goto, Yasuyuki and Sanjoba, Chizu and Endo, Yasuyuki and Fujiyuki, Tomoko and Sugai, Akihiro and Tsukiyama-Kohara, Kyoko and Matsumoto, Yoshitsugu and Sato, Hiroki and Kai, Chieko},
 issue = {7},
 journal = {PLoS Neglected Tropical Diseases},
 month = {Jul},
 note = {Article, Canine distemper virus (CDV) vaccination confers long-term protection against CDV reinfection. To investigate the utility of CDV as a polyvalent vaccine vector for Leishmania, we generated recombinant CDVs, based on an avirulent Yanaka strain, that expressed Leishmania antigens: LACK, TSA, or LmSTI1 (rCDV–LACK, rCDV–TSA, and rCDV–LmSTI1, respectively). Dogs immunized with rCDV-LACK were protected against challenge with lethal doses of virulent CDV, in the same way as the parental Yanaka strain. To evaluate the protective effects of the recombinant CDVs against cutaneous leishmaniasis in dogs, dogs were immunized with one recombinant CDV or a cocktail of three recombinant CDVs, before intradermal challenge (in the ears) with infective-stage promastigotes of Leishmania major. Unvaccinated dogs showed increased nodules with ulcer formation after 3 weeks, whereas dogs immunized with rCDV–LACK showed markedly smaller nodules without ulceration. Although the rCDV–TSA- and rCDV–LmSTI1-immunized dogs showed little protection against L. major, the cocktail of three recombinant CDVs more effectively suppressed the progression of nodule formation than immunization with rCDV–LACK alone. These results indicate that recombinant CDV is suitable for use as a polyvalent live attenuated vaccine for protection against both CDV and L. major infections in dogs.},
 pages = {e0003914-1--e0003914-15},
 title = {Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs},
 volume = {9},
 year = {2015}
}