{"created":"2023-06-19T06:59:26.931078+00:00","id":369,"links":{},"metadata":{"_buckets":{"deposit":"2365ef72-f981-4082-8adb-8c8240643413"},"_deposit":{"created_by":3,"id":"369","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"369"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00000369","sets":["9:10:11:14"]},"author_link":["1225","1224","1226","1227","1223"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"174","bibliographicPageStart":"169","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"Zygote"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The phenomenon of developmental arrest at the 2-cell stage of zygotes obtained from certain mouse strains during in vitro  culture is known as the 2-cell block. The effect of conditioned medium (CM) with rat hepatoma BRL cells on the 2-cell block of CD-1 mouse zygotes was investigated in comparison with that of CM with rat hepatoma Reuber H-35 cells. In control medium with EDTA, 75.4% of 2-cell embryos developed to the 4- to 8-cell stages. In the same conditions, the BRL Mr  <10000 fraction inhibited the development of 2-cell embryos to the 4- to 8-cell stages (57.7%), although the inhibition by this fraction was weaker than by the Reuber Mr <10000 fraction (19.8%). As a result of reversed-phase column chromatography, a 2-cell stage specific inhibitor of the cleavage of mouse embryos (Fr.B-25), which separated into the Mr <10000 fraction of the Reuber CM, was detected at a low level in the BRL Mr <10000 fraction. On the other hand, the Mr  >10000 fraction of BRL CM accelerated the development of the embryos (90.3%). This beneficial effect was also evident even in the absence of EDTA. RT-PCR analysis revealed that mRNAs encoding the β-A or β-B subunit of activins (Mr ~29000), which are well characterized cytokines that act as releasers of the 2-cell block, were expressed in BRL cells. These results indicate that BRL cells synthesize Fr.B-25 at low levels, and that activins contained in the BRL CM probably contributed to overcoming the 2-cell block of CD-1 zygotes cultured in vitro.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Cambridge University Press"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1017/S0967199408005121","subitem_relation_type_select":"DOI"}}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"http://dx.doi.org/10.1017/S0967199408005121 | http://dx.doi.org/10.1017/S0967199408005121"}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0967-1994","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kobayashi, Masayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Terawaki, Yoshinori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saito, Koichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kasuga, Kano"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kojima, Ikuo"}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effect of medium conditioned with rat hepatoma BRL cells on '2-cell block' of random-bred mouse embryos cultured in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of medium conditioned with rat hepatoma BRL cells on '2-cell block' of random-bred mouse embryos cultured in vitro"}]},"item_type_id":"2","owner":"3","path":["14"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-06-14"},"publish_date":"2011-06-14","publish_status":"0","recid":"369","relation_version_is_last":true,"title":["Effect of medium conditioned with rat hepatoma BRL cells on '2-cell block' of random-bred mouse embryos cultured in vitro"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T09:21:30.449396+00:00"}