@article{oai:rakuno.repo.nii.ac.jp:00000408,
 author = {Kang, Sung-Sik and Ofuji, Sosuke and Imai, Kei and Huang, Weiping and Koyama, Keisuke and Yanagawa, Yojiro and Takahashi, Yoshiyuki and Nagano, Masashi},
 issue = {3},
 journal = {zygote},
 month = {Jun},
 note = {Article, The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.},
 pages = {412--415},
 title = {The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development},
 volume = {23},
 year = {2015}
}