{"created":"2023-06-19T07:02:57.479420+00:00","id":5166,"links":{},"metadata":{"_buckets":{"deposit":"c636cf5f-e3fd-4b9f-b2c9-cda5598a516d"},"_deposit":{"created_by":3,"id":"5166","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5166"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00005166","sets":["9:10:11:14"]},"author_link":["16027","16028","16029","16026","16023"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"40","bibliographicPageEnd":"20931","bibliographicPageStart":"20924","bibliographicVolumeNumber":"291","bibliographic_titles":[{"bibliographic_title":"The Journal of Biological Chemistry"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Genomic imprinting is an epigenetic mechanism that switches the expression of imprinted genes involved in normal embryonic growth and development in a parent-of-origin-specific manner. Changes in DNA methylation statuses from polyploidization are a well characterized epigenetic modification in plants. However, how changes in ploidy affect both imprinted gene expression and methylation status in mammals remains unclear. To address this, we used quantitative real time PCR to analyze expression levels of imprinted genes in mouse tetraploid fetuses. We used bisulfite sequencing to assess the methylation statuses of differentially methylated regions (DMRs) that regulate imprinted gene expression in triploid and tetraploid fetuses. The nine imprinted genes H19, Gtl2, Dlk1, Igf2r, Grb10, Zim1, Peg3, Ndn, and Ipw were all unregulated; in particular, the expression of Zim1 was more than 10-fold higher, and the expression of Ipw was repressed in tetraploid fetuses. The methylation statuses of four DMRs H19, intergenic (IG), Igf2r, and Snrpn in tetraploid and triploid fetuses were similar to those in diploid fetuses. We also performed allele-specific RT-PCR sequencing to determine the alleles expressing the three imprinted genes Igf2, Gtl2, and Dlk1 in tetraploid fetuses. These three imprinted genes showed monoallelic expression in a parent-of-origin-specific manner. Expression of non-imprinted genes regulating neural cell development significantly decreased in tetraploid fetuses, which might have been associated with unregulated imprinted gene expression. This study provides the first detailed analysis of genomic imprinting in tetraploid fetuses, suggesting that imprinted gene expression is disrupted, but DNA methylation statuses of DMRs are stable following changes in ploidy in mammals.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology"}]},"item_2_relation_11":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"1083-351X","subitem_relation_type_select":"ISBN"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1074/jbc.M116.744144","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This research was originally published in The Journal of Biological Chemistry. Wataru Yamazaki, Tomoko Amano, Hanako Bai, Masashi Takahashi and Manabu Kawahara. The Influence of Polyploidy and Genome Composition on Genomic Imprinting in Mice. The Journal of Biological Chemistry. 2016; Vol: 291(40) 20924-20931. © the American Society for Biochemistry and Molecular Biology."}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamazaki, Wataru"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Amano, Tomoko","creatorNameLang":"en"},{"creatorName":"天野, 朋子","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Bai, Hanako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawahara, Manabu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-12"}],"displaytype":"detail","filename":"R-2017-95_Amano.pdf","filesize":[{"value":"698.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2017-95_Amano.pdf","url":"https://rakuno.repo.nii.ac.jp/record/5166/files/R-2017-95_Amano.pdf"},"version_id":"0546ffc9-762a-4a24-ad99-f4b3181bfe90"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Influence of Polyploidy and Genome Composition on Genomic Imprinting in Mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Influence of Polyploidy and Genome Composition on Genomic Imprinting in Mice"}]},"item_type_id":"2","owner":"3","path":["14"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-12"},"publish_date":"2018-04-12","publish_status":"0","recid":"5166","relation_version_is_last":true,"title":["The Influence of Polyploidy and Genome Composition on Genomic Imprinting in Mice"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-30T01:47:11.969243+00:00"}