{"created":"2023-06-19T07:02:57.735338+00:00","id":5170,"links":{},"metadata":{"_buckets":{"deposit":"218b2d6f-7b57-4441-be1c-3a500e8800fc"},"_deposit":{"created_by":3,"id":"5170","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5170"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00005170","sets":["9:10:11:14"]},"author_link":["16049","16057","16062","16056","16063","16061","16065","16058","16064","16055","16059","16060"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-02","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"21411-14","bibliographicPageStart":"21411-1","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"RNA viruses use various strategies to condense their genetic information into small genomes. Potyviruses not only use the polyprotein strategy, but also embed an open reading frame, pipo, in the P3 cistron in the -1 reading frame. PIPO is expressed as a fusion protein with the N-terminal half of P3 (P3N-PIPO) via transcriptional slippage of viral RNA-dependent RNA polymerase (RdRp). We herein show that clover yellow vein virus (ClYVV) produces a previously unidentified factor, P3N-ALT, in the +1 reading frame via transcriptional slippage at a conserved G_<1-2>A_<6-7> motif, as is the case for P3N-PIPO. The translation of P3N-ALT terminates soon, and it is considered to be a C-terminal truncated form of P3. In planta experiments indicate that P3N-ALT functions in cell-to-cell movement along with P3N-PIPO. Hence, all three reading frames are used to produce functional proteins. Deep sequencing of ClYVV RNA from infected plants endorses the slippage by viral RdRp. Our findings unveil a virus strategy that optimizes the coding capacity.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Macmillan Publishers Limited"}]},"item_2_relation_11":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"2045-2322","subitem_relation_type_select":"ISBN"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1038/srep21411","subitem_relation_type_select":"DOI"}}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Hagiwara-Komoda, Yuka","creatorNameLang":"en"},{"creatorName":"薦田, 優香","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Choi, Sun Hee"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Masanao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Atsumi, Go"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Abe, Junya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuda, Junya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Honjo, Mie N."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagano, Atsushi J."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Komoda, Keisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakahara, Kenji S."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uyeda, Ichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Naito, Satoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-12"}],"displaytype":"detail","filename":"R-2017-101_Komoda.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2017-101_Komoda.pdf","url":"https://rakuno.repo.nii.ac.jp/record/5170/files/R-2017-101_Komoda.pdf"},"version_id":"84c84dbc-cb79-41be-89f3-e1e94a450882"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses"}]},"item_type_id":"2","owner":"3","path":["14"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-12"},"publish_date":"2018-04-12","publish_status":"0","recid":"5170","relation_version_is_last":true,"title":["Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-30T01:42:00.496647+00:00"}