{"created":"2023-06-19T07:02:58.781944+00:00","id":5186,"links":{},"metadata":{"_buckets":{"deposit":"74b96907-45ff-4faa-8dc6-d73214ba5d29"},"_deposit":{"created_by":3,"id":"5186","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5186"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00005186","sets":["9:10:18:19"]},"author_link":["16145","16144","2908","16146","16142","16143"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"32","bibliographicPageEnd":"16447","bibliographicPageStart":"16438","bibliographicVolumeNumber":"291","bibliographic_titles":[{"bibliographic_title":"The Journal of Biological Chemistry"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The actinobacterium Kribbella flavida NBRC 14399^T produces cyclobis-(1→6)-α-nigerosyl (CNN), a cyclic glucotetraose with alternate α-(1→6)- and α-(1→3)-glucosidic linkages, from starch in the culture medium. We identified gene clusters associated with the production and intracellular catabolism of CNN in the K. flavida genome. One cluster encodes 6-α-glucosyltransferase and 3-α-isomaltosyltransferase, which are known to coproduce CNN from starch. The other cluster contains four genes annotated as a transcriptional regulator, sugar transporter, glycoside hydrolase family (GH) 31 protein (Kfla1895), and GH15 protein (Kfla1896). Kfla1895 hydrolyzed the α-(1→3)-glucosidic linkages of CNN and produced isomaltose via a possible linear tetrasaccharide. The initial rate of hydrolysis of CNN (11.6 s^<−1>) was much higher than that of panose (0.242 s^<−1>), and hydrolysis of isomaltotriose and nigerose was extremely low. Because Kfla1895 has a strong preference for the α-(1→3)-isomaltosyl moiety and effectively hydrolyzes the α-(1→3)-glucosidic linkage, it should be termed 1,3-α-isomaltosidase. Kfla1896 effectively hydrolyzed isomaltose with liberation of β-glucose, but displayed low or no activity toward CNN and the general GH15 enzyme substrates such as maltose, soluble starch, or dextran. The k_<cat>/K_m for isomaltose (4.81 ± 0.18^<s−1> mm^<−1>) was 6.9- and 19-fold higher than those for panose and isomaltotriose, respectively. These results indicate that Kfla1896 is a new GH15 enzyme with high substrate specificity for isomaltose, suggesting the enzyme should be designated an isomaltose glucohydrolase. This is the first report to identify a starch-utilization pathway that proceeds via CNN. ","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology"}]},"item_2_relation_11":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"1083-351X","subitem_relation_type_select":"ISBN"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1074/jbc.M116.727305","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This research was originally published in The Journal of Biological Chemistry. Takayoshi Tagami, Eri Miyano, Juri Sadahiro, Masayuki Okuyama, Tomohito Iwasaki and Atsuo Kimura. Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl. The Journal of Biological Chemistry. 2016; 291(32): 16438-16447. © the American Society for Biochemistry and Molecular Biology."}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tagami, Takayoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyano, Eri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sadahiro, Juri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okuyama, Masayuki"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"IWASAKI, Tomohito","creatorNameLang":"en"},{"creatorName":"岩﨑, 智仁","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kimura, Atsuo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-12"}],"displaytype":"detail","filename":"R-2017-145_Iwasaki.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2017-145_Iwasaki.pdf","url":"https://rakuno.repo.nii.ac.jp/record/5186/files/R-2017-145_Iwasaki.pdf"},"version_id":"916f87a6-e2aa-4044-a4ef-997de42bc967"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl"}]},"item_type_id":"2","owner":"3","path":["19"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-12"},"publish_date":"2018-04-12","publish_status":"0","recid":"5186","relation_version_is_last":true,"title":["Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T00:49:35.485469+00:00"}