{"created":"2023-06-19T07:03:00.194769+00:00","id":5208,"links":{},"metadata":{"_buckets":{"deposit":"c7a58538-1178-4c39-b7e9-aae6d5d7aed2"},"_deposit":{"created_by":3,"id":"5208","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5208"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00005208","sets":["9:27:28:29"]},"author_link":["16281","16278","16277","16279","7686","16280","16276"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"46-12","bibliographicPageStart":"46-1","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"Viruses"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Dengue virus (DENV) infection causes one of the most widespread mosquito-borne diseases in the world. Despite the great need, effective vaccines and practical antiviral therapies are still under development. Intracellular lipid levels are regulated by sterol regulatory elements-binding proteins (SREBPs), which are activated by serine protease, site 1 protease (S1P). Small compound PF-429242 is known as a S1P inhibitor and the antivirus effects have been reported in some viruses. In this study, we examined the anti-DENV effects of PF-429242 using all four serotypes of DENV by several primate-derived cell lines. Moreover, emergence of drug-resistant DENV mutants was assessed by sequential passages with the drug. DENV dependency on intracellular lipids during their infection was also evaluated by adding extracellular lipids. The addition of PF-429242 showed suppression of viral propagation in all DENV serotypes. We showed that drug-resistant DENV mutants are unlikely to emerge after five times sequential passages through treatment with PF-429242. Although the levels of intracellular cholesterol and lipid droplets were reduced by PF-429242, viral propagations were not recovered by addition of exogenous cholesterol or fatty acids, indicating that the reduction of LD and cholesterol caused by PF-429242 treatment is not related to its mechanism of action against DENV propagation. Our results suggest that PF-429242 is a promising candidate for an anti-DENV agent.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI AG"}]},"item_2_relation_11":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"1999-4915","subitem_relation_type_select":"ISBN"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.3390/v8020046","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license."}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by/4.0/ | https://creativecommons.org/licenses/by/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"内田, 玲麻","creatorNameLang":""}],"familyNames":[{"familyNameLang":""}],"givenNames":[{"givenNameLang":""}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Urata, Shuzo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ulanday, Gianne Eduard L."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takamatsu, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuda, Jiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayasaka, Daisuke"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-12"}],"displaytype":"detail","filename":"R-2017-226_Uchida.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2017-226_Uchida.pdf","url":"https://rakuno.repo.nii.ac.jp/record/5208/files/R-2017-226_Uchida.pdf"},"version_id":"d7a5eee2-787d-4e5f-8312-5d7bba29c9c9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"dengue virus","subitem_subject_scheme":"Other"},{"subitem_subject":"antivirus drug","subitem_subject_scheme":"Other"},{"subitem_subject":"PF-429242","subitem_subject_scheme":"Other"},{"subitem_subject":"SREBPs","subitem_subject_scheme":"Other"},{"subitem_subject":"S1P","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Suppressive Effects of the Site 1 Protease (S1P) Inhibitor, PF-429242, on Dengue Virus Propagation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Suppressive Effects of the Site 1 Protease (S1P) Inhibitor, PF-429242, on Dengue Virus Propagation"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-12"},"publish_date":"2018-04-12","publish_status":"0","recid":"5208","relation_version_is_last":true,"title":["Suppressive Effects of the Site 1 Protease (S1P) Inhibitor, PF-429242, on Dengue Virus Propagation"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T02:06:43.042496+00:00"}